Note: Raman microspectroscopy integrated with fluorescence and dark field imaging

被引：22

作者：

Li, Haibo ^{[1
]}

Wang, Hailong ^{[1
]}

Huang, Dianshuai ^{[2
]}

Liang, Lijia ^{[1
]}

Gu, Yuejiao ^{[1
]}

Liang, Chongyang ^{[2
]}

Xu, Shuping ^{[1
]}

Xu, Weiqing ^{[1
]}

机构：

[1] Jilin Univ, State Key Lab Supramol Struct & Mat, Changchun 130012, Peoples R China

[2] Jilin Univ, Inst Frontier Med Sci, Changchun 130021, Peoples R China

来源：

REVIEW OF SCIENTIFIC INSTRUMENTS | 2014年 / 85卷 / 05期

基金：

中国国家自然科学基金;

关键词：

SINGLE-MOLECULE; SPECTROSCOPY; SCATTERING; CELL;

D O I：

10.1063/1.4878715

中图分类号：

TH7 [仪器、仪表];

学科分类号：

0804 ; 080401 ; 081102 ;

摘要：

A Raman detection platform integrated with both fluorescence and dark field microscopes was built for in situ Raman detection with the assistance of fluorescence and dark field imaging to locate the target micro regions. Cells and organelles can be easily found via fluorescence imaging with labeling techniques. Besides, nano-sized particles could be observed and located by dark field microscopes. Therefore, comparing with the commercial Raman spectrometers, much more researches based on Raman spectroscopy could be carried out on this integrated Raman platform, especially in the fields of analyzing biological tissues and subwavelength samples. (C) 2014 AIP Publishing LLC.

引用

页数：3

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