Dodecafluoroheptanol: Oxygen reservoir for the culture of mouse melanoma B16 cells

被引：6

作者：

Miyajima, Hiroki ^{[1
]}

Kasuya, Maria Camelita ^{[1
]}

Hatanaka, Kenichi ^{[1
]}

机构：

[1] Univ Tokyo, Inst Ind Sci, Meguro Ku, Tokyo 1538505, Japan

来源：

JOURNAL OF FLUORINE CHEMISTRY | 2014年 / 163卷

关键词：

Fluorous; Melanoma B16 cells; Dissolved oxygen; Biphasic culture; DEPENDENT MAMMALIAN-CELLS; LIQUID-LIQUID INTERFACES; DISSOLVED-OXYGEN; PERFLUOROCARBON; GROWTH; SYSTEM;

D O I：

10.1016/j.jfluchem.2014.04.010

中图分类号：

O61 [无机化学];

学科分类号：

070301 ; 081704 ;

摘要：

Mouse melanoma B16 cells thrived during culture at the interphase between the aqueous culture medium and the fluorous solvent layers. However, cell spreading was not observed. Cells were rounded and attached to each other forming a cell sheet. Cells consumed the dissolved oxygen in the aqueous medium during culture and the depleted amount of oxygen was replenished in the presence of dodecafluoroheptanol. This research demonstrated that the fluorous solvent layer could serve as an oxygen reservoir for biphasic animal cell culture. (C) 2014 Elsevier B.V. All rights reserved.

引用

页码：46 / 49

页数：4

共 18 条

[1]

ANDO J, 1991, IN VITRO CELL DEV B, V27, P525

[2] ENHANCEMENT OF OXYGEN-TRANSFER IN HYBRIDOMA CELL-CULTURE BY USING A PERFLUOROCARBON AS AN OXYGEN CARRIER [J].

CHO, MH ;

WANG, SS .

BIOTECHNOLOGY LETTERS, 1988, 10 (12) :855-860

[3]

Davis J.M., 1996, BASIC CELL CULTURE P, V2nd

[4] Long-term continuous monitoring of dissolved oxygen in cell culture medium for perfused bioreactors using optical oxygen sensors [J].

Gao, FG ;

Jeevarajan, AS ;

Anderson, MM .

BIOTECHNOLOGY AND BIOENGINEERING, 2004, 86 (04) :425-433

[5] BEHAVIOR OF CELLS AT FLUID INTERFACES [J].

GIAEVER, I ;

KEESE, CR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1983, 80 (01) :219-222

[6] Fluorous solvent for cell culture [J].

Kasuya, Maria Carmelita Z. ;

Wen, Xiaonan ;

Hatanaka, Kenichi ;

Akashi, Kageyasu .

JOURNAL OF FLUORINE CHEMISTRY, 2011, 132 (11) :978-981

[7] Dissolved oxygen concentration in serum-free continuous culture affects N-linked glycosylation of a monoclonal antibody [J].

Kunkel, JP ;

Jan, DCH ;

Jamieson, JC ;

Butler, M .

JOURNAL OF BIOTECHNOLOGY, 1998, 62 (01) :55-71

[8]

Kwon YJ, 2001, BIOTECHNOL BIOENG, V72, P331, DOI 10.1002/1097-0290(20010205)72:3<331::AID-BIT10>3.0.CO

[9]

2-A

[10] Culture of cells at perfluorocarbon-aqueous interfaces [J].

Lowe, KC ;

Anthony, P ;

Davey, MR ;

Power, JB .

ARTIFICIAL CELLS BLOOD SUBSTITUTES AND IMMOBILIZATION BIOTECHNOLOGY, 1999, 27 (03) :255-261

← 1 2 →