Evaluation of plant cell wall degrading enzyme production by Clostridium thermocellum B8 in the presence of raw agricultural wastes

被引：13

作者：

Hamann, Pedro Ricardo V. ^{[1
]}

Serpa, Dayane L. ^{[1
]}

Barreto da Cunha, Amanda Souza ^{[1
]}

de Camargo, Brenda R. ^{[1
]}

Osiro, Karen Ofuji ^{[1
]}

de Sousa, Marcelo Valle ^{[2
]}

Felix, Carlos R. ^{[1
]}

Miller, Robert N. G. ^{[3
]}

Noronha, Eliane F. ^{[1
]}

机构：

[1] Univ Brasilia, Dept Cell Biol, Enzymol Lab, Brasilia, DF, Brazil

[2] Univ Brasilia, Dept Cellular Biol, Lab Biochem & Prot Chem, Brasilia, DF, Brazil

[3] Univ Brasilia, Dept Cellular Biol, Brasilia, DF, Brazil

来源：

INTERNATIONAL BIODETERIORATION & BIODEGRADATION | 2015年 / 105卷

关键词：

Clostridium thermocellum; Holocellulases; Agro-industrial wastes; Biofuel; AGROINDUSTRIAL RESIDUES; LIGNOCELLULOSIC BIOMASS; ATCC; 27405; PURIFICATION; BIOCONVERSION; EXPRESSION; XYLANASE; FERMENTATION; CELLULOSE; PROTEINS;

D O I：

10.1016/j.ibiod.2015.08.013

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Plant cell wall represents an important source of fermentable sugars for second generation bioethanol production. However, cellulosic biomass hydrolysis still is a bottleneck to bioethanol production in an efficient and low cost process. Thermophilic bacteria have been studied as a source of cellulolytic enzymes for cellulosic biomass deconstruction, as their enzymes present unique features compatible with current industrial process conditions. The present study was carried out to evaluate the use of different agro-industrial wastes as suitable carbon sources for growth and enzyme secretion by a strain of Clostridium thermocellum isolated from goat rumen. C thermocellum BS was able to grow on/degrade microcrystalline cellulose, Sugar cane bagasse/Straw and Cotton waste, and produced different sets of cellulases and hemicellulases in their presence. The enzymatic mixtures produced by C. thermocellum (BS) showed activities over a broad range of temperatures (50-70 degrees C). Highest values were obtained between 60 and 70 degrees C, at a pH range from 5 to 7, decreasing at alkaline pH values. In addition, enzymes displayed thermostability, with CMCase and xylanase activities maintaining maximum values over 12 days at 50 degrees C. (C) 2015 Elsevier Ltd. All rights reserved.

引用

页码：97 / 105

页数：9

共 40 条

[1] CHARACTERIZATION AND PURIFICATION OF THERMOSTABLE BETA-GLUCOSIDASE FROM CLOSTRIDIUM THERMOCELLUM [J].

AIT, N ;

CREUZET, N ;

CATTANEO, J .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1979, 90 (02) :537-546

[2] Production of fibrolytic enzymes by Aspergillus japonicus C03 using agro-industrial residues with potential application as additives in animal feed [J].

Antonio Facchini, Fernanda Dell ;

Vici, Ana Claudia ;

Amin Reis, Victor Ricardo ;

Jorge, Joao Atilio ;

Terenzi, Hector Francisco ;

Reis, Ricardo Andrade ;

Teixeira de Moraes Polizeli, Maria de Lourdes .

BIOPROCESS AND BIOSYSTEMS ENGINEERING, 2011, 34 (03) :347-355

[3] IMPROVED SILVER STAINING OF PLANT-PROTEINS, RNA AND DNA IN POLYACRYLAMIDE GELS [J].

BLUM, H ;

BEIER, H ;

GROSS, HJ .

ELECTROPHORESIS, 1987, 8 (02) :93-99

[4]

Blume L.R., 2011, THESIS U BRASILA BR

[5] Characterization of Clostridium thermocellum Isolates Grown on Cellulose and Sugarcane Bagasse [J].

Blume, Liana R. ;

Noronha, Eliane F. ;

Leite, Jackeline ;

Queiroz, Rayner M. L. ;

Ornelas Ricart, Carlos A. ;

de Sousa, Marcelo Valle ;

Felix, Carlos R. .

BIOENERGY RESEARCH, 2013, 6 (02) :763-775

[6] Thermophilic lignocellulose deconstruction [J].

Blumer-Schuette, Sara E. ;

Brown, Steven D. ;

Sander, Kyle B. ;

Bayer, Edward A. ;

Kataeva, Irina ;

Zurawski, Jeffrey V. ;

Conway, Jonathan M. ;

Adams, Michael W. W. ;

Kelly, Robert M. .

FEMS MICROBIOLOGY REVIEWS, 2014, 38 (03) :393-448

[7]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[8] Weedy lignocellulosic feedstock and microbial metabolic engineering: advancing the generation of 'Biofuel' [J].

Chandel, Anuj K. ;

Singh, Om V. .

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2011, 89 (05) :1289-1303

[9] Bacteria and Archaea community structure in the rumen microbiome of goats (Capra hircus) from the semiarid region of Brazil [J].

Cunha, Isabel S. ;

Barreto, Cristine C. ;

Costa, Ohana Y. A. ;

Bomfim, Marco A. ;

Castro, Alinne P. ;

Kruger, Ricardo H. ;

Quirino, Betania F. .

ANAEROBE, 2011, 17 (03) :118-124

[10] Metagenomic Insights into the Fibrolytic Microbiome in Yak Rumen [J].

Dai, Xin ;

Zhu, Yaxin ;

Luo, Yingfeng ;

Song, Lei ;

Liu, Di ;

Liu, Li ;

Chen, Furong ;

Wang, Min ;

Li, Jiabao ;

Zeng, Xiaowei ;

Dong, Zhiyang ;

Hu, Songnian ;

Li, Lingyan ;

Xu, Jian ;

Huang, Li ;

Dong, Xiuzhu .

PLOS ONE, 2012, 7 (07)

← 1 2 3 4 →