Identification of aerobic-denitrifying Psychrobacter cryohalolentis strain F5-6 and its nitrate removal at low temperature

被引：5

作者：

Hou, Yue ^{[1
,2
]}

Zhang, Duoying ^{[3
,5
]}

Cao, Haoran ^{[3
]}

Zhang, Yanlong ^{[1
,2
]}

Zhao, Dandan ^{[1
,2
]}

Zeng, Weimin ^{[1
,2
]}

Lei, Hong ^{[1
,2
]}

Bai, Ye ^{[4
]}

机构：

[1] Heilongjiang Univ, Engn Res Ctr Agr Microbiol Technol, Minist Educ, Harbin 150500, Peoples R China

[2] Heilongjiang Univ, Coll Heilongjiang Prov, Sch Life Sci, Key Lab Microbiol, Harbin 150080, Peoples R China

[3] Heilongjiang Univ, Sch Civil Engn, Harbin 150080, Peoples R China

[4] Boli Cty Market Supervis Adm, Inspection & Test Ctr, Qitaihe 154500, Peoples R China

[5] Heilongjiang Univ, Sch Civil Engn, 74 Xuefu Rd, Harbin 150080, Peoples R China

来源：

INTERNATIONAL BIODETERIORATION & BIODEGRADATION | 2022年 / 172卷

基金：

中国国家自然科学基金;

关键词：

Psychrophilic bacteria; Denitrification; Nitrate; Psychrobacter cryohalolentis; Cold adaptation; Kinetics; DOMESTIC WASTE-WATER; HETEROTROPHIC NITRIFICATION; AMMONIUM REMOVAL; SP NOV; DENITRIFICATION; BACTERIUM; NITROGEN; PERFORMANCE; PHOSPHORUS; GENES;

D O I：

10.1016/j.ibiod.2022.105426

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Biological denitrification is suppressed when temperature is lower than 10 degrees C. In this study, Psychrobacter cryohalolentis F5-6 was isolated from Harbin region and showed high aerobic-denitrifying rate at low temperature. It contained high percentage of unsaturated fatty acids (87.32%) and short chain saturated acid (3.4% C10:0 and 4.0% C12:0 3-OH). Acetate was the best carbon source for the strain F5-6 performing denitrification. Aerobic denitrification (napA gene) and nitrate assimilation (nasA gene) were the nitrate metabolism processes for the strain F5-6. Its biological nitrate removal (BNR) rate at 10 degrees C showed fit with Monod equation (R2 = 0.9808). The maximum BNR rate was 23.71 mg NO3- -N g- 1DW h-1 and KS was 116.96 mg L-1. The optimal denitrifying condition was pH 7.5, 15 degrees C with shaking speed of 180 rpm and C/N of 2. Results of this study supplied a bacterial resource for improving nitrate removal from low C/N water at low temperature.

引用

页数：8