Identification of aerobic-denitrifying Psychrobacter cryohalolentis strain F5-6 and its nitrate removal at low temperature

被引:5
|
作者
Hou, Yue [1 ,2 ]
Zhang, Duoying [3 ,5 ]
Cao, Haoran [3 ]
Zhang, Yanlong [1 ,2 ]
Zhao, Dandan [1 ,2 ]
Zeng, Weimin [1 ,2 ]
Lei, Hong [1 ,2 ]
Bai, Ye [4 ]
机构
[1] Heilongjiang Univ, Engn Res Ctr Agr Microbiol Technol, Minist Educ, Harbin 150500, Peoples R China
[2] Heilongjiang Univ, Coll Heilongjiang Prov, Sch Life Sci, Key Lab Microbiol, Harbin 150080, Peoples R China
[3] Heilongjiang Univ, Sch Civil Engn, Harbin 150080, Peoples R China
[4] Boli Cty Market Supervis Adm, Inspection & Test Ctr, Qitaihe 154500, Peoples R China
[5] Heilongjiang Univ, Sch Civil Engn, 74 Xuefu Rd, Harbin 150080, Peoples R China
基金
中国国家自然科学基金;
关键词
Psychrophilic bacteria; Denitrification; Nitrate; Psychrobacter cryohalolentis; Cold adaptation; Kinetics; DOMESTIC WASTE-WATER; HETEROTROPHIC NITRIFICATION; AMMONIUM REMOVAL; SP NOV; DENITRIFICATION; BACTERIUM; NITROGEN; PERFORMANCE; PHOSPHORUS; GENES;
D O I
10.1016/j.ibiod.2022.105426
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Biological denitrification is suppressed when temperature is lower than 10 degrees C. In this study, Psychrobacter cryohalolentis F5-6 was isolated from Harbin region and showed high aerobic-denitrifying rate at low temperature. It contained high percentage of unsaturated fatty acids (87.32%) and short chain saturated acid (3.4% C10:0 and 4.0% C12:0 3-OH). Acetate was the best carbon source for the strain F5-6 performing denitrification. Aerobic denitrification (napA gene) and nitrate assimilation (nasA gene) were the nitrate metabolism processes for the strain F5-6. Its biological nitrate removal (BNR) rate at 10 degrees C showed fit with Monod equation (R2 = 0.9808). The maximum BNR rate was 23.71 mg NO3- -N g- 1DW h-1 and KS was 116.96 mg L-1. The optimal denitrifying condition was pH 7.5, 15 degrees C with shaking speed of 180 rpm and C/N of 2. Results of this study supplied a bacterial resource for improving nitrate removal from low C/N water at low temperature.
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页数:8
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