Possible dysregulation of chaperon and metabolic proteins in cystic fibrosis bronchial tissue

被引:28
作者
Frischer, Thomas [1 ]
Myung, Jae-Kyung [1 ]
Maurer, Gerald [1 ]
Eichler, Irmgard [1 ]
Szepfalusi, Zsolt [1 ]
Lubec, Gert [1 ]
机构
[1] Med Univ Vienna, Dept Pediat, Vienna, Austria
关键词
bronchial tissue; cystic fibrosis; mass spectrometry; protein derangement; two-dimensional gel electrophoresis;
D O I
10.1002/pmic.200500487
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Cystic fibrosis (CF) is an autosomal recessive disease due to mutations of the CF transmembrane conductance regulator gene. A systematic approach to generate a protein expressional pattern in CF bronchial tissue has not been performed so far. It was the aim of this hypothesis-generating study to construct differential proteomes of bronchial biopsies in controls (n = 8) and CF patients (n = 9). Biopsies (pools of three per patient) were taken; proteins were extracted and run on 2-DE with subsequent in-gel digestion and mass spectrometrical identification and quantification of proteins using specific software. Three hundred sixty-six protein spots were identified and compared between groups. Following an approach for multiple testing correction, the chaperone 75 kDa glucose-regulated protein and ubiquinol-cytochrome c reductase complex core protein I and one form of nidogen, a pseudogene of aconitase 2, were increased in CF (p < 0.005). Aberrant protein levels may reflect molecular changes of CF as well as CF-linked inflammation, infection and cellular stress response.
引用
收藏
页码:3381 / 3388
页数:8
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