Topographic mapping in dorsoventral axis of the xenopus retinotectal system depends on signaling through ephrin-13 ligands

被引：163

作者：

Mann, F ^{[1
]}

Ray, S ^{[1
]}

Harris, WA ^{[1
]}

Holt, CE ^{[1
]}

机构：

[1] Univ Cambridge, Dept Anat, Cambridge CB2 3DY, England

来源：

NEURON | 2002年 / 35卷 / 03期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1016/S0896-6273(02)00786-9

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Ephrin-B and EphB are distributed in matching dorsoventral gradients in the embryonic Xenopus visual system with retinal axons bearing high levels of ligand (dorsal) projecting to tectal regions with high receptor expression (ventral). In vitro stripe assays show that dorsal retinal axons prefer to grow on EphB receptor stripes supporting an attractive guidance mechanism. In vivo disruption of EphB/ephrin-B function by application of exogenous EphB or expression of dominant-negative ephrin-B ligand in dorsal retinal axons causes these axons to shift dorsally in the tectum, while misexpression of wild-type ephrin-B in ventral axons causes them to shift ventrally. These dorsoventral targeting errors are consistent with the hypothesis that an attractive mechanism that requires ephrin-B cytoplasmic domain is critical for retinotectal mapping in this axis.

引用

页码：461 / 473

页数：13

共 69 条

[1] The cytoplasmic domain of the ligand ephrinB2 is required for vascular morphogenesis but not cranial neural crest migration [J].