Green Synthesis of Silver Nanoparticles from Cleome Viscosa: Synthesis and Antimicrobial Activity

The synthesis of metal and semiconductor nanoparticles is an expanding research area due to the potential applications for the development of novel technologies. In this work, we describe a cost effective and environment friendly technique for green synthesis of silver phyto nanoparticles and their antibiogram from 3mM silver nitrate solution through the extract of Cleome viscosa as reducing as well as capping agent. In the process of synthesising silver nanoparticles we observed a rapid reduction of silver ions leading to the formation of stable crystalline silver nanoparticles in the solution. The herbal leaves and their medicinal properties were already discussed in varieties of ayurvedic studies.The synthesis of silver phyto nanoparticles were prepared by adding silver nitrate solution solution [3mM] to the plant extract. Nanoparticles were characterised using UV-Visible absorption spectroscopy, FTIR, XRD, XRF,TEM AND SEM analysis. The biomass of plants produces their nanomaterials by a process called bio mineralisation. The silver phyto nanoparticles were isolated from these herbal leaves and tested for antimicrobial activity. The test cultures included in this study were Staphylococcus aureus, E.Coli, Pseudomonas aeruginosa, Vibrio cholerae, and Salmonella. The maximum inhibitory effect using 3mM silver nitrate against the microbes were obtained.