Simple and sensitive determination of 5-fluorouracil in plasma by high-performance liquid chromatography -: Application to clinical pharmacokinetic studies

被引:30
作者
Escoriaza, J
Aldaz, A
Calvo, E
Giráldez, J
机构
[1] Univ Navarra Clin, Serv Farm, Pamplona 31008, Spain
[2] Univ Navarra Clin, Dept Oncol, Pamplona 31008, Spain
来源
JOURNAL OF CHROMATOGRAPHY B | 1999年 / 736卷 / 1-2期
关键词
5-fluorouracil;
D O I
10.1016/S0378-4347(99)00446-6
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
5-Fluorouracil (5-FU) is an antineoplastic agent widely employed in the treatment of many types of cancer. Recent studies have proved the need for individual adjustment of 5-FU dosage based on pharmacokinetics. A simple and sensitive high-performance liquid chromatographic method for the determination of 5-FU in plasma and their preliminary clinical pharmacokinetics is described. After sample acidification with 20 mu l of orthophosphoric acid (5%), the drug is extracted from plasma using n-propanol-diethyl ether (16:84). The organic layer is evaporated to dryness, the residue dissolved in 100 mu l of mobile phase and 20 mu l of this mixture is injected into a LiChrospher 100RP-18 (5 mu m, 250x4.0 mm) analytical column. Mobile phase consisted of potassium dihydrogenphosphate (0.05 M, adjusted to pH 3). The limit of quantitation was 2 ng/ml. The method showed good precision: the within-day relative standard deviation (RSD) for 5-FU (10-20 000 ng/ml) was 3.75% (2.57-5.93); the between-day RSD for 5-FU, in the previously described range, was 5.74% (4.35-7.20). The method presented here is accurate, precise and sensitive and it has been successfully applied for 5-FU pharmacokinetic investigation and therapeutic drug monitoring. (C) 1999 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:97 / 102
页数:6
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