Cardiotoxicity evaluation using human embryonic stem cells and induced pluripotent stem cell-derived cardiomyocytes

被引：24
|
作者：
Zhao, Qi [1 ]
Wang, Xijie [1 ]
Wang, Shuyan [1 ]
Song, Zheng [1 ]
Wang, Jiaxian [2 ]
Ma, Jing [1 ]
机构：
[1] Natl Shanghai Ctr New Drug Safety Evaluat & Res, China State Inst Pharmaceut Ind, Shanghai, Peoples R China
[2] Nanjing Med Univ, Affiliated Hosp 1, Dept Cardiol, Nanjing, Jiangsu, Peoples R China
来源：
STEM CELL RESEARCH & THERAPY | 2017年 / 8卷
关键词：
Stem cells; Cardiomyocytes; Cardiotoxicity; Pharmacology; DE-POINTES; LONG-QT; HALOPERIDOL; PROLONGATION; MAGNESIUM; ARRAYS; MODEL;
D O I：
10.1186/s13287-017-0473-x
中图分类号：
Q813 [细胞工程];
学科分类号：
摘要：
Background: Cardiotoxicity remains an important concern in drug discovery. Human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) have become an attractive platform to evaluate cardiotoxicity. However, the consistency between human embryonic stem cell-derived cardiomyocytes (hESC-CMs) and human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) in prediction of cardiotoxicity has yet to be elucidated. Methods: Here we screened the toxicities of four representative drugs (E-4031, isoprenaline, quinidine, and haloperidol) using both hESC-CMs and hiPSC-CMs, combined with an impedance-based bioanalytical method. Results: It showed that both hESC-CMs and hiPSC-CMs can recapitulate cardiotoxicity and identify the effects of well-characterized compounds. Conclusions: The combined platform of hPSC-CMs and an impedance-based bioanalytical method could improve preclinical cardiotoxicity screening, holding great potential for increasing drug development accuracy.
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页数：7
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