Nutrient deprivation increases vulnerability of endothelial cells to proinflammatory insults

被引:19
作者
Russell, Fraser D. [1 ]
Hamilton, Karina D. [1 ]
机构
[1] Univ Sunshine Coast, Sch Hlth & Sport Sci, Maroochydore, Qld 4558, Australia
关键词
Reactive oxygen species; Superoxide; HUVECs; Serum deprivation; Tumor necrosis factor-alpha; Free radicals; DENSITY-LIPOPROTEIN RECEPTOR-1; OXYGEN SPECIES GENERATION; OXIDATIVE STRESS; NADPH OXIDASE; LECTIN-LIKE; APOPTOSIS; SUPEROXIDE; AUTOPHAGY; GLUCOSE; DYSFUNCTION;
D O I
10.1016/j.freeradbiomed.2013.12.007
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Nutrient deprivation is a stimulus for oxidative stress and is an established, method for induction of cell autophagy and apoptosis. The aims of this study were to identify conditions that evoke superoxide production in cultured human umbilical vein endothelial cells (HUVECs), determine the mechanism of action for this response, and examine whether the stimulus might facilitate the adhesion of human isolated neutrophils to the HUVECs. HUVECs were incubated in M199 medium under conditions of serum starvation (serum-free M199 medium), low serum (medium containing 2% fetal calf serum), and high serum (medium containing 20% fetal calf serum). HUVECs were also incubated under proinflammatory conditions, in medium supplemented with 50 ng/ml tumor necrosis factor-alpha (TNF-alpha) or neutrophils preactivated with 10 nM phorbol 12-myristate 13-acetate (PMA). Superoxide production was increased fourfold in serum-starved HUVECs compared to cells incubated in 20% medium, and this was reduced by inhibitors of the mitochondrial electron transport chain and mitochondrial Ca2+ uniporter. Superoxide production was 23.6% higher in HUVECs incubated with TNF-alpha in 2% medium compared to 2% medium alone, but unchanged with TNF-alpha in 20% medium. PMA-activated neutrophils adhered to morphologically aberrant HUVECs, which were mainly evident under the low-serum condition. The findings show a role of mitochondrial enzymes in superoxide production in response to nutrient deprivation and suggest that proinflammatory responses in HUVECs become manifest when HUVECs are in an already-compromised state. (C) 2013 Elsevier Inc. All rights reserved.
引用
收藏
页码:408 / 415
页数:8
相关论文
共 43 条
  • [1] Bachurin SO, 1996, NEUROTOXICOLOGY, V17, P897
  • [2] Regulation of reactive oxygen species generation in cell signaling
    Bae, Yun Soo
    Oh, Hyunjin
    Rhee, Sue Goo
    Do Yoo, Young
    [J]. MOLECULES AND CELLS, 2011, 32 (06) : 491 - 509
  • [3] A protocol for isolation and culture of human umbilical vein endothelial cells
    Baudin, Bruno
    Bruneel, Arnaud
    Bosselut, Nelly
    Vaubourdolle, Michel
    [J]. NATURE PROTOCOLS, 2007, 2 (03) : 481 - 485
  • [4] Exogenous BH4/Bcl-2 peptide reverts coronary endothelial cell apoptosis induced by oxidative stress
    Cantara, S
    Donnini, S
    Giachetti, A
    Thorpe, PE
    Ziche, M
    [J]. JOURNAL OF VASCULAR RESEARCH, 2004, 41 (02) : 202 - 207
  • [5] EVALUATION OF TRIACETYLOLEANDOMYCIN, ALPHA-NAPHTHOFLAVONE AND DIETHYLDITHIOCARBAMATE AS SELECTIVE CHEMICAL PROBES FOR INHIBITION OF HUMAN CYTOCHROMES P450
    CHANG, TKH
    GONZALEZ, FJ
    WAXMAN, DJ
    [J]. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1994, 311 (02) : 437 - 442
  • [6] Superoxide is the major reactive oxygen species regulating autophagy
    Chen, Y.
    Azad, M. B.
    Gibson, S. B.
    [J]. CELL DEATH AND DIFFERENTIATION, 2009, 16 (07) : 1040 - 1052
  • [7] Oxidative stress and endothelial dysfunction in cerebrovascular disease
    Chrissobolis, Sophocles
    Miller, Alyson A.
    Drummond, Grant R.
    Kemp-Harper, Barbara K.
    Sobey, Christopher G.
    [J]. FRONTIERS IN BIOSCIENCE-LANDMARK, 2011, 16 : 1733 - 1745
  • [8] Induction of apoptosis by high proinsulin and glucose in cultured human umbilical vein endothelial cells is mediated by reactive oxygen species
    Du, XL
    Sui, GZ
    Stockklauser-Färber, K
    Weiss, J
    Zink, S
    Schwippert, B
    Wu, QX
    Tschöpe, D
    Rösen, P
    [J]. DIABETOLOGIA, 1998, 41 (03) : 249 - 256
  • [9] Expression, Regulation and Putative Nutrient-Sensing Function of Taste GPCRs in the Heart
    Foster, Simon R.
    Porrello, Enzo R.
    Purdue, Brooke
    Chan, Hsiu-Wen
    Voigt, Anja
    Frenzel, Sabine
    Hannan, Ross D.
    Moritz, Karen M.
    Simmons, David G.
    Molenaar, Peter
    Roura, Eugeni
    Boehm, Ulrich
    Meyerhof, Wolfgang
    Thomas, Walter G.
    [J]. PLOS ONE, 2013, 8 (05):
  • [10] Frey RS, 2009, ANTIOXID REDOX SIGN, V11, P791, DOI [10.1089/ars.2008.2220, 10.1089/ARS.2008.2220]