Effects of instilled combustion-derived particles in spontaneously hypertensive rats. Part II: Pulmonary responses

被引:11
作者
Wichers, LB
Nolan, JP
Winsett, DW
Ledbetter, AD
Kodavanti, UP
Schladweiler, MCJ
Costa, DL
Watkinson, WP
机构
[1] US EPA, Pulm Toxicol Branch, Expt Toxicol Div, Natl Hlth & Environm Effects Res Lab,Off Res & De, Res Triangle Pk, NC 27711 USA
[2] Univ N Carolina, Sch Publ Hlth, Dept Environm Sci & Engn, Chapel Hill, NC 27599 USA
关键词
D O I
10.1080/08958370490439704
中图分类号
R99 [毒物学(毒理学)];
学科分类号
100405 ;
摘要
A consistent association between exposure to high concentrations of ambient particulate matter (PM) and excess cardiopulmonary-related morbidity and mortality has been observed in numerous epidemiological studies, across many different geographical locations. To elicit a similar response in a controlled laboratory setting, spontaneously hypertensive rats were exposed to an oil combustion-derived PM (HP-12) and monitored for changes in pulmonary function and indices of pulmonary injury. Rats were implanted with radiotelemeters to monitor electrocardiogram, heart rate, systemic arterial blood pressure, core temperature, and activity. Animals were divided into four groups and exposed via intratracheal instillation (IT) to suspensions of HP-12 (0.0, 0.83, 3.33, and 8.33 mg/kg; control, low, mid, and high dose, respectively) in saline vehicle. Telemetered rats were monitored continuously for 4-7 days post-IT and pulmonary function was examined using a whole-body plethysmograph system for 6 h/day on post-IT days 1-7. At 24, 96, and 192 h post-IT, bronchoalveolar lavage fluid (BALF) was obtained from subsets of nontelemetered animals in order to assess the impact of HP-12 on biochemical indices of pulmonary inflammation and injury. Immediate dose-related changes in pulmonary function were observed after HP-12 exposure, consisting of decreases in tidal volume (down arrow12-41%) and increases in breathing frequency (up arrow52-103%), minute ventilation (up arrow12-25%), and enhanced pause (up arrow113-187%). These functional effects were resolved by 7 days post-IT, although some average BALF constituents remained elevated through day 7 for mid- and high-dose groups when compared to those of the saline-treated control group. This study demonstrates significant deficits in pulmonary function, along with significant increases in BALF indices of pulmonary inflammation and injury in SH rats after IT exposure to HP-12.
引用
收藏
页码:407 / 419
页数:13
相关论文
共 48 条
[1]   Lung function and long term exposure to air pollutants in Switzerland [J].
AckermannLiebrich, U ;
Leuenberger, P ;
Schwartz, J ;
Schindler, C ;
Monn, C ;
Bolognini, C ;
Bongard, JP ;
Brandli, O ;
Domenighetti, G ;
Elsasser, S ;
Grize, L ;
Karrer, W ;
Keller, R ;
KellerWossidlo, H ;
Kunzli, N ;
Martin, BW ;
Medici, TC ;
Perruchoud, AP ;
Schoni, MH ;
Tschopp, JM ;
Villiger, B ;
Wuthrich, B ;
Zellweger, JP ;
Zemp, E .
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, 1997, 155 (01) :122-129
[2]   Zinc is the toxic factor in the lung response to an atmospheric particulate sample [J].
Adamson, IYR ;
Prieditis, H ;
Hedgecock, C ;
Vincent, R .
TOXICOLOGY AND APPLIED PHARMACOLOGY, 2000, 166 (02) :111-119
[3]   Respiratory effects of relocating to areas of differing air pollution levels [J].
Avol, EL ;
Gauderman, WJ ;
Tan, SM ;
London, SJ ;
Peters, JM .
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, 2001, 164 (11) :2067-2072
[4]   Cardiac and thermoregulatory effects of instilled particulate matter-associated transition metals in healthy and cardiopulmonary-compromised rats [J].
Campen, MJ ;
Nolan, JP ;
Schladweiler, MCJ ;
Kodavanti, UP ;
Costa, DL ;
Watkinson, WP .
JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES, 2002, 65 (20) :1615-1631
[5]   Cardiovascular and thermoregulatory effects of inhaled PM-associated transition metals: A potential interaction between nickel and vanadium sulfate [J].
Campen, MJ ;
Nolan, JP ;
Schladweiler, MCJ ;
Kodavanti, UP ;
Evansky, PA ;
Costa, DL ;
Watkinson, WP .
TOXICOLOGICAL SCIENCES, 2001, 64 (02) :243-252
[6]  
Campen MJ, 2000, INHAL TOXICOL, V12, P7, DOI 10.1080/08958378.2000.11463196
[7]   PULMONARY-FUNCTION AND AMBIENT PARTICULATE MATTER - EPIDEMIOLOGIC EVIDENCE FROM NHANES-I [J].
CHESTNUT, LG ;
SCHWARTZ, J ;
SAVITZ, DA ;
BURCHFIEL, CM .
ARCHIVES OF ENVIRONMENTAL HEALTH, 1991, 46 (03) :135-144
[8]  
COSTA DL, 1986, LAB ANIM SCI, V36, P256
[9]   Bioavailable transition metals in particulate matter mediate cardiopulmonary injury in healthy and compromised animal models [J].
Costa, DL ;
Dreher, KL .
ENVIRONMENTAL HEALTH PERSPECTIVES, 1997, 105 :1053-1060
[10]   Ambient particle inhalation and the cardiovascular system: Potential mechanisms [J].
Donaldson, K ;
Stone, V ;
Seaton, A ;
MacNee, W .
ENVIRONMENTAL HEALTH PERSPECTIVES, 2001, 109 :523-527