Droplet array on local redox cycling-based electrochemical (LRC-EC) chip device

被引：14

作者：

Ino, Kosuke ^{[1
]}

Goto, Takehito ^{[1
]}

Kanno, Yusuke ^{[1
]}

Inoue, Kumi Y. ^{[1
]}

Takahashi, Yasufumi ^{[1
]}

Shiku, Hitoshi ^{[1
]}

Matsue, Tomokazu ^{[1
,2
]}

机构：

[1] Tohoku Univ, Grad Sch Environm Studies, Sendai, Miyagi 980, Japan

[2] Tohoku Univ, WPI Adv Inst Mat Res, Sendai, Miyagi 980, Japan

来源：

LAB ON A CHIP | 2014年 / 14卷 / 04期

基金：

日本科学技术振兴机构; 日本学术振兴会;

关键词：

MICROELECTRODE ARRAY; CELLS; ELECTRODES; MICROSCOPY; INTERFACES;

D O I：

10.1039/c3lc51156a

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We have previously reported a local redox cycling-based electrochemical (LRC-EC) system for the incorporation of many electrochemical sensors into a small chip device. In the present study, a new type of LRC-EC chip device was fabricated for the detection of a droplet array. To detect electrochemically redox compounds in droplets, Pt pseudo-reference/counter electrodes were incorporated into the individual sensors of the LRC-EC chip device. Cyclic voltammetry for the LRC-EC chip device with internal Pt pseudo-reference electrodes indicated well-defined voltammograms based on redox cycling for the individual sensor points. The device was successfully applied to the addressable detection of alkaline phosphatase (ALP) activity of HeLa cells in single droplets on the sensor points. Therefore, the LRC-EC chip device is considered to be a useful device for the bioanalysis of droplet systems.

引用

页码：787 / 794

页数：8

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