Interaction between the critical aromatic amino acid residues Tyr352 and Phe504 in the yeast Ga12 transporter

被引：6

作者：

Kasahara, T ^{[1
]}

Kasahara, M ^{[1
]}

机构：

[1] Teikyo Univ, Sch Med, Biophys Lab, Hachioji, Tokyo 1920395, Japan

来源：

FEBS LETTERS | 2000年 / 471卷 / 01期

关键词：

galactose transport; Ga12; Hxt2; substrate recognition; glucose transport; aromatic amino acid;

D O I：

10.1016/S0014-5793(00)01371-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Three critical aromatic sites have been identified in the yeast galactose transporter Gal2: Tyr(352) at the extracellular boundary of putative transmembrane segment (TM) 7, Tyr(446) in the middle of TM10 and Phe(504) in the middle of TM12. The relationship between these sites was investigated by random mutagenesis of each combination of two of the three residues. Galactose transport-positive clones selected by plate assays encoded Tyr(446) and specific combinations of aromatic residues at sites 352 and 504. Double-site mutants containing aromatic residues at these latter two positions showed either essentially full galactose transport activity (Phe(352)Trp(504) and Trp(352)Trp(504)) Or no significant activity (Phe(352)Tyr(504) and Trp(352)Tyr(504)), whereas single-site mutants showed markedly; reduced activity. These results are indicative of a specific interaction between sites 352 and 504 of Gal2, (C) 2000 Federation of European Biochemical Societies.

引用

页码：103 / 107

页数：5

共 25 条

[1] MAMMALIAN PASSIVE GLUCOSE TRANSPORTERS - MEMBERS OF AN UBIQUITOUS FAMILY OF ACTIVE AND PASSIVE TRANSPORT PROTEINS [J].

BALDWIN, SA .

BIOCHIMICA ET BIOPHYSICA ACTA, 1993, 1154 (01) :17-49

[2] YEAST SUGAR TRANSPORTERS [J].

BISSON, LF ;

COONS, DM ;

KRUCKEBERG, AL ;

LEWIS, DA .

CRITICAL REVIEWS IN BIOCHEMISTRY AND MOLECULAR BIOLOGY, 1993, 28 (04) :259-308

[3] The molecular genetics of hexose transport in yeasts [J].

Boles, E ;

Hollenberg, CP .

FEMS MICROBIOLOGY REVIEWS, 1997, 21 (01) :85-111

[4] The aromatic residues Trp and Phe have different effects on the positioning of a transmembrane helix in the microsomal membrane [J].

Braun, P ;

von Heijne, G .

BIOCHEMISTRY, 1999, 38 (30) :9778-9782

[5] Crystal structures of various maltooligosaccharides bound to maltoporin reveal a specific sugar translocation pathway [J].

Dutzler, R ;

Wang, YF ;

Rizkallah, PJ ;

Rosenbusch, JP ;

Schirmer, T .

STRUCTURE, 1996, 4 (02) :127-134

[6] Structure of the sucrose-specific porin ScrY from Salmonella typhimurium and its complex with sucrose [J].

Forst, D ;

Welte, W ;

Wacker, T ;

Diederichs, K .

NATURE STRUCTURAL BIOLOGY, 1998, 5 (01) :37-46

[7] Automated protein sequence database classification. I. Integration of compositional similarity search, local similarity search, and multiple sequence alignment [J].

Gracy, J ;

Argos, P .

BIOINFORMATICS, 1998, 14 (02) :164-173

[8] Contribution to substrate recognition of two aromatic amino acid residues in putative transmembrane segment 10 of the yeast sugar transporters Gal2 and Hxt2 [J].

Kasahara, M ;

Maeda, M .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (44) :29106-29112

[9] Transmembrane segment 10 is important for substrate recognition in Ga12 and Hxt2 sugar transporters in the yeast Saccharomyces cerevisiae [J].

Kasahara, M ;

Shimoda, E ;

Maeda, M .

FEBS LETTERS, 1996, 389 (02) :174-178

[10] Amino acid residues responsible for galactose recognition in yeast Gal2 transporter [J].

Kasahara, M ;

Shimoda, E ;

Maeda, M .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (27) :16721-16724

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