Bienzyme sensors based on novel polymethylferrocenyl dendrimers

被引：35
作者：
Losada, J.
Zamora, M.
Garcia Armada, P.
Cuadrado, I.
Alonso, B.
Casado, C. M.
机构：
[1] Univ Politecn Madrid, Dpto Ingn Quim Ind, ETSIL, E-28006 Madrid, Spain
[2] Univ Autonoma Madrid, Fac Ciencias, Dpto Quim Inorgan, E-28049 Madrid, Spain
来源：
ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2006年 / 385卷 / 07期
关键词：
polymethylferrocene; dendrimers; bienzyme electrodes; horseradish peroxidase (HRP); glucose oxidase; amperometric biosensor;
D O I：
10.1007/s00216-006-0330-8
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Amperometric bienzyme electrodes with horseradish peroxidase (HRP) and glucose oxidase (GOx) co-immobilized on polymethylferrocenyl dendrimers deposited onto platinum electrodes have been used for determination of the hydrogen peroxide produced by the oxidase during the enzymatic reaction. The redox dendrimers consist of flexible poly(propylenimine) dendrimer cores functionalised with octamethylferrocenyl units. The effects of dendrimer generation, the thickness of the dendrimer layer, substrate concentration, interferences, and reproducibility on the response of the sensors were investigated. The new bienzyme biosensors respond to substrate at work potential values between 200 and 50 mV (vs. SCE), have good sensitivity, and are resistant to interferences.
引用
收藏
页码：1209 / 1217
页数：9
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