Capturing protein-coding genes across highly divergent species

被引：153

作者：

Li, Chenhong ^{[1
,3
]}

Hofreiter, Michael ^{[2
]}

Straube, Nicolas ^{[3
]}

Corrigan, Shannon ^{[3
]}

Naylor, Gavin J. P. ^{[3
]}

机构：

[1] Coll Charleston, Hollings Marine Lab, Charleston, SC 29401 USA

[2] Univ York, Dept Biol, Heslington, N Yorkshire, England

[3] Coll Charleston, Hollings Marine Lab, Charleston, SC 29401 USA

来源：

BIOTECHNIQUES | 2013年 / 54卷 / 06期

基金：

美国国家科学基金会;

关键词：

comparative biology; cross-species capture; DNA hybridization capture; next-generation sequencing; protein-coding genes; phylogenetics; target enrichment; ULTRACONSERVED ELEMENTS; EXON CAPTURE; MITOCHONDRIAL; EVOLUTION; HYBRIDIZATION; MARKERS; SEARCH; GENOME;

D O I：

10.2144/000114039

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

DNA hybridization capture combined with next generation sequencing can be used to determine the sequences of hundreds of target genes across hundreds of individuals in a single experiment. However, the approach has thus far only been successfully applied to capture targets that are highly similar in sequence to the bait molecules. Here we introduce modifications that extend the reach of the method to allow efficient capture of highly divergent homologous target sequences using a single set of baits. These modifications have important implications for comparative biology.

引用

页码：321 / +

页数：5

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