Amperometric algal Chlorella vulgaris cell biosensors based on alginate and polypyrrole-alginate gels

被引：50
作者：
Ionescu, Rodica E.
Abu-Rabeah, Khalil
Cosnier, Serge
Durrieu, Claude
Chovelon, Jean-Marc
Marks, Robert S.
机构：
[1] Univ Grenoble 1, CNRS, FR 2607, Inst Chim Mol Grenoble,UMR 5630,Lab Electrochim O, F-38041 Grenoble 9, France
[2] Ben Gurion Univ Negev, Dept Biotechnol Engn, IL-84105 Beer Sheva, Israel
[3] ENTPE, Lab Sci Environm, F-69518 Vaulx En Velin, France
[4] Univ Lyon 1, UMR 5634, Lab Applicat Chim Environm, F-69100 Villeurbanne, France
[5] Natl Inst Biotechnol Negev, IL-84105 Beer Sheva, Israel
来源：
ELECTROANALYSIS | 2006年 / 18卷 / 11期
关键词：
algae; Chlorella vulgaris; alginate; pyrrole-alginate; amperometry;
D O I：
10.1002/elan.200603506
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
The successful development and analytical performances of two biosensor configurations based on the entrapment of algal cells of Chlorella vulgaris into either a regular alginate gel or a newly synthesized pyrrole-alginate matrix are reported. These biosensors were compared in terms of their amperometric current measurements to p-nitrophenyl phosphate when used as substrate for the detection of an algal alkaline phosphatase activity. The high stability of the pyrrole-alginate gel when compared to that of the alginate coating is herein demonstrated.
引用
收藏
页码：1041 / 1046
页数：6
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