Norcantharidin Suppresses YD-15 Cell Invasion Through Inhibition of FAK/Paxillin and F-Actin Reorganization

被引：15

作者：

Hong, Kyoung-Ok ^{[1
,2
]}

Ahn, Chi-Hyun ^{[1
,2
]}

Yang, In-Hyoung ^{[1
,2
]}

Han, Jung-Min ^{[1
,2
]}

Shin, Ji-Ae ^{[1
,2
]}

Cho, Sung-Dae ^{[1
]}

Hong, Seong Doo ^{[1
]}

机构：

[1] Seoul Natl Univ, Sch Dent, Dept Oral Pathol, Seoul 03080, South Korea

[2] Seoul Natl Univ, Dent Res Inst, Seoul 03080, South Korea

来源：

MOLECULES | 2019年 / 24卷 / 10期

基金：

新加坡国家研究基金会;

关键词：

mucoepidermoid carcinoma; norcantharidin; invasion; F-actin reorganization; focal adhesion kinase; paxillin; FOCAL ADHESION KINASE; MUCOEPIDERMOID CARCINOMA; IN-VITRO; CANCER; FAK; PROGRESSION; HALLMARKS; PRODUCTS; VINCULIN;

D O I：

10.3390/molecules24101928

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Norcantharidin (NCTD), a demethylated derivative of cantharidin, has been reported to exhibit activity against various types of cancers. However, the anti-invasive effects of NCTD and its molecular mechanism in human mucoepidermoid carcinoma (MEC) remain incompletely elucidated. Clonogenic, wound healing, invasion, zymography, western blotting and immunocytochemistry assays were performed in YD-15 cells to investigate the anti-invasive effect of NCTD and its molecular mechanism of action. The inhibitory effects of NCTD on invasiveness were compared with those of a novel focal adhesion kinase (FAK) kinase inhibitor, PF-562271. NCTD markedly suppressed the colony formation, migration, and invasion of YD-15 cells as well as the activities of MMP-2 and MMP-9. It disrupted F-actin reorganization through suppressing the FAK/Paxillin axis. Moreover, NCTD exhibited a powerful anti-invasive effect compared with that of PF-562271 in YD-15 cells. Collectively, these results suggest that NCTD has a potential anti-invasive activity against YD-15 cells. This study may clarify the impact of NCTD on migration and invasion of human MEC cells.

引用

页数：11