glp-3 is required for mitosis and meiosis in the Caenorhabditis elegans germ line

被引:0
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作者
Kadyk, LC
Lambie, EJ
Kimble, J
机构
[1] UNIV WISCONSIN,MOL BIOL LAB,HOWARD HUGHES MED INST,MADISON,WI 53706
[2] UNIV WISCONSIN,DEPT BIOCHEM,MADISON,WI 53706
[3] UNIV WISCONSIN,DEPT MED GENET,MADISON,WI 53706
[4] DARTMOUTH COLL,HANOVER,NH 03755
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中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
The germ line is the only tissue in Caenorhabditis elegans in which a stem cell population continues to divide mitotically throughout life; hence the cell cycles of the germ line and the soma are regulated differently. Here we report the genetic and phenotypic characterization of the glp-3 gene. In animals homozygous for each of five recessive loss-of-function alles, germ cells in both hermaphrodites and males fail to progress through mitosis and meiosis, but somatic cells appear to divide normally. Germ cells in animals grown at 15 degrees appear by DAPI staining to be uniformly arrested at the G2/M transition with <20 germ cells per gonad on average, suggesting a checkpoint-mediated arrest. In contrast, germ cells in mutant animals grown at 25 degrees frequently proliferate slowly during adulthood, eventually forming small germ lines with several hundred germ cells. Nevertheless, cells in these small germ lines never undergo meiosis. Double mutant analysis with mutations in other genes affecting germ cell proliferation supports the idea that glp-3 may encode a gene product that is required for the mitotic and meiotic cell cycles in the C. elegans germ line.
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页码:111 / 121
页数:11
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