Enzymatic deacylation of 1,2-diacyl-sn-glycero-3-phosphocholines to sn-glycerol-3-phosphocholine

被引：15

作者：

Blasi, F. ^{[1
]}

Cossignani, L. ^{[1
]}

Simonetti, M. S. ^{[1
]}

Brutti, M. ^{[1
]}

Ventura, F. ^{[1
]}

Damiani, P. ^{[1
]}

机构：

[1] Univ Perugia, Dept Econ Sci & Food Sci, Sect Food Chem & Anal, DSEEA, I-06126 Perugia, Italy

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 2006年 / 39卷 / 07期

关键词：

1,2-diacyl-sn-glycero-3-phosphocholine; sn-glycerol-3-phosphocholine; lipozyme IM lipase; phospholipase A2;

D O I：

10.1016/j.enzmictec.2006.03.026

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

This research was undertaken to study the enzymatic deacylation of 1,2-diacyl-sn-glycero-3-phosphocholines (sn-1,2-PC) to sn-glycerol-3-phosphocholine (GPC); this compound could be an useful intermediate in the synthesis of "structured" sn-1,2-PC, after re-acylations of the two sn-positions of the glycerol backbone. The enzymatic reactions represent a valid alternative to the chemical deacylation that can be simply obtained in alkaline conditions. High conversion were achieved using a lipase selective for the sn-1-position of sn-1,2-PC (Lipozyme IM, from Mucor miehei) together with a Phospholipase A(2) from hog pancreas, enzyme selective for the sn-2-position; the best results were obtained carrying out the enzymatic reaction in a microemulsion system. (c) 2006 Elsevier Inc. All rights reserved.

引用

页码：1405 / 1408

页数：4

共 15 条

[1] Synthesis of phosphatidylcholine with defined fatty acid in the sn-1 position by lipase-catalyzed esterification and transesterification reaction
Adlercreutz, D
Budde, H
Wehtje, E
[J]. BIOTECHNOLOGY AND BIOENGINEERING, 2002, 78 (04) : 403 - 411
[2] [Anonymous], STRUCTURAL MODIFIED
[3] SIMPLIFIED PREPARATION OF L-ALPHA-GLYCERYL PHOSPHORYL CHOLINE
BROCKERHOFF, H
YURKOWSKI, M
[J]. CANADIAN JOURNAL OF BIOCHEMISTRY, 1965, 43 (10): : 1777 - +
[4] Characterization and optimization of phospholipase A2 catalyzed synthesis of phosphatidylcholine
Egger, D
Wehtje, E
Adlercreutz, P
[J]. BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY, 1997, 1343 (01): : 76 - 84
[5] Gunstone FD, 1999, J SCI FOOD AGR, V79, P1535, DOI 10.1002/(SICI)1097-0010(199909)79:12<1535::AID-JSFA430>3.0.CO
[6] 2-7
[7] Comparative study of commercially available lipases in hydrolysis reaction of phosphatidylcholine
Hara, F
Nakashima, T
Fukuda, H
[J]. JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, 1997, 74 (09) : 1129 - 1132
[8] Study of TAG ethanolysis to 2-MAG by immobilized Candida antarctica lipase and synthesis of symmetrically structured TAG
Irimescu, R
Iwasaki, Y
Hou, CT
[J]. JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, 2002, 79 (09) : 879 - 883
[9] Enzymatic synthesis of structured lipids
Iwasaki, Y
Yamane, T
[J]. JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 2000, 10 (1-3) : 129 - 140
[10] Lipase-catalyzed synthesis of lysophosphatidylcholine using organic cosolvent for in situ water activity control
Kim, L
Kim, BG
[J]. JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, 2000, 77 (07) : 791 - 797

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