Purification of glycyrrhizin from Glycyrrhiza uralensis Fisch with ethanol/phosphate aqueous two phase system

被引：92

作者：

Tan, TW ^{[1
]}

Huo, Q

Ling, Q

机构：

[1] Beijing Univ Chem Technol, Dept Biochem Engn, Beijing 100029, Peoples R China

[2] Beijing United Univ, Dept Chem Engn, Beijing 100029, Peoples R China

来源：

BIOTECHNOLOGY LETTERS | 2002年 / 24卷 / 17期

关键词：

aqueous two phase system; ethanol/phosphate system; Glycyrrhiza uralensis Fisch; glycyrrhizin; separation;

D O I：

10.1023/A:1019850531640

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Phase diagrams of alcohol (ethanol or 2-propanol)/salt (phosphate or sulfate) aqueous two-phase systems were made. The system consisting of 60% (v/v) ethanol and 15% (w/v) phosphate was then used to separate glycyrrhizin from an extract of Glycyrrhiza uralensis Fisch and gave a 92% recovery of glycyrrhizin with 2.6-fold purification.

引用

页码：1417 / 1420

页数：4

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