Role of Kif15 and its novel mitotic partner KBP in K-fiber dynamics and chromosome alignment

被引：20
作者：
Brouwers, Nathalie [1 ,2 ]
Mallol Martinez, Nuria [1 ,2 ]
Vernos, Isabelle [1 ,2 ,3 ]
机构：
[1] Barcelona Inst Sci & Technol, Cell & Dev Biol Program, CRG, Dr Aiguader 88, Barcelona, Spain
[2] UPF, Barcelona, Spain
[3] ICREA, Pg Lluis Co 23, Barcelona, Spain
来源：
PLOS ONE | 2017年 / 12卷 / 04期
关键词：
KINESIN-LIKE PROTEIN; CENTROSOME SEPARATION; MICROTUBULE DYNAMICS; KINETOCHORE FIBERS; SPINDLE POLES; LOCALIZATION; CONGRESSION; DYNEIN; CELLS; MITOCHONDRIA;
D O I：
10.1371/journal.pone.0174819
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Faithful segregation of the genetic material during the cell cycle is key for the continuation of life. Central to this process is the assembly of a bipolar spindle that aligns the chromosomes and segregates them to the two daughter cells. Spindle bipolarity is strongly dependent on the activity of the homotetrameric kinesin Eg5. However, another kinesin, Kif15, also provides forces needed to separate the spindle poles during prometaphase and to maintain spindle bipolarity at metaphase. Here we identify KBP as a specific interaction partner of Kif15 in mitosis. We show that KBP promotes the localization of Kif15 to the spindle equator close to the chromosomes. Both Kif15 and KBP are required for the alignment of all the chromosomes to the metaphase plate and the assembly of stable kinetochore fibers of the correct length. Taken together our data uncover a novel role for Kif15 in complex with KBP during mitosis.
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页数：21
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