Downregulation of the long noncoding RNA SNHG1 inhibits tumor cell migration and invasion by sponging miR-195 through targeting Cdc42 in oesophageal cancer

被引：14
作者：
Chen, Yu [1 ,2 ]
Sheng, Hong-Guang [3 ]
Deng, Fu-Mou [3 ]
Cai, Li-Ly [1 ,2 ]
机构：
[1] Jiangxi Prov Key Lab Lab Med, Nanchang, Jiangxi, Peoples R China
[2] Nanchang Univ, Affiliated Hosp 2, Dept Clin Lab, 1 Minde Rd, Nanchang 330006, Jiangxi, Peoples R China
[3] Nanchang Univ, Affiliated Hosp 2, Dept Anesthesiol, Nanchang, Jiangxi, Peoples R China
来源：
KAOHSIUNG JOURNAL OF MEDICAL SCIENCES | 2021年 / 37卷 / 03期
关键词：
invasion; lncRNA SNHG1; migration; miR‐ 195; oesophageal cancer; LNCRNA SNHG1; METASTASIS; EXPRESSION; PROLIFERATION; CARCINOMA; PROGRESSION; CONTRIBUTES;
D O I：
10.1002/kjm2.12318
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
Despite the poor prognosis of oesophageal cancer (EC), the molecular mechanisms of EC are still unclear. In recent years, role of lncRNA in cancer development attracted much attention. The present study aimed to investigate the effects of the long noncoding RNA SNHG1 on the migration and invasion of EC cells and the possible mechanisms involved. The effects of SNHG1 on cell proliferation, migration, and invasion were determined and its relationship with miR-195/Cdc42 axis was investigated. It was found SNHG1 and Cdc42 were significantly upregulated, and miR-195 was significantly downregulated in both EC tissues and cell lines. In addition, the inhibition of either SNHG1 or Cdc42 resulted in suppression of cell proliferation, migration, and invasion, while inhibition of miR-195 led to opposite results and reversed the effects of si-SNHG1. We also observed that higher SNHG1 predicted poorer prognosis of EC patients. In summary, inhibition of SNHG1 can suppress the cell migration and invasion of EC cells by sponging miR-195 through targeting Cdc42. This study might provide deeper insights into the SNHG1/miR-195/Cdc42 axis in EC.
引用
收藏
页码：181 / 191
页数：11
相关论文
共 27 条
[1]   Transducer of Cdc42-dependent actin assembly promotes breast cancer invasion and metastasis [J].
Chander, H. ;
Truesdell, P. ;
Meens, J. ;
Craig, A. W. B. .
ONCOGENE, 2013, 32 (25) :3080-3090
[2]  
Chen XJ, 2015, AM J CANCER RES, V5, P2808
[3]   CD90+liver cancer cells modulate endothelial cell phenotype through the release of exosomes containing H19 lncRNA [J].
Conigliaro, Alice ;
Costa, Viviana ;
Lo Dico, Alessia ;
Saieva, Laura ;
Buccheri, Simona ;
Dieli, Francesco ;
Manno, Mauro ;
Raccosta, Samuele ;
Mancone, Carmine ;
Tripodi, Marco ;
De Leo, Giacomo ;
Alessandro, Riccardo .
MOLECULAR CANCER, 2015, 14
[4]   Upregulated lncRNA SNHG1 contributes to progression of nonsmall cell lung cancer through inhibition of miR-101-3p and activation of Wnt/β-catenin signaling pathway [J].
Cui, Yun ;
Zhang, Fuming ;
Zhu, Chunkai ;
Geng, Liang ;
Tian, Tongde ;
Liu, Huaimin .
ONCOTARGET, 2017, 8 (11) :17785-17794
[5]   The functional role of long non-coding RNA in human carcinomas [J].
Gibb, Ewan A. ;
Brown, Carolyn J. ;
Lam, Wan L. .
MOLECULAR CANCER, 2011, 10
[6]   LncRNA-SNHG1 contributes to gastric cancer cell proliferation by regulating DNMT1 [J].
Hu, Yongbo ;
Ma, Zhen ;
He, Yiming ;
Liu, Wei ;
Su, Yu ;
Tang, Zongbin .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2017, 491 (04) :926-931
[7]   Prognostic Implications of MicoRNA miR-195 Expression in Human Tongue Squamous Cell Carcinoma [J].
Jia, Ling-fei ;
Wei, Su-bi ;
Gong, Kai ;
Gan, Ye-hua ;
Yu, Guang-yan .
PLOS ONE, 2013, 8 (02)
[8]   lncRNA-UCA1 enhances cell proliferation through functioning as a ceRNA of Sox4 in esophageal cancer [J].
Jiao, Changjie ;
Song, Zhiming ;
Chen, Jianming ;
Zhong, Jing ;
Cai, Weibin ;
Tian, Suke ;
Chen, Songlin ;
Yi, Yunfeng ;
Xiao, Yun .
ONCOLOGY REPORTS, 2016, 36 (05) :2960-2966
[9]   SNHG1 lncRNA negatively regulates rriiR-199a-3p to enhance CDK7 expression and promote cell proliferation in prostate cancer [J].
Li, Jianping ;
Zhang, Zhipeng ;
Xiong, Li ;
Guo, Chuan ;
Jiang, Tao ;
Zeng, Lilan ;
Li, Ge ;
Wang, Juan .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2017, 487 (01) :146-152
[10]   LncRNA &ITSNHG1&IT enhances cell proliferation, migration, and invasion in cervical cancer [J].
Liu, Yang ;
Yang, Yanling ;
Li, Lei ;
Liu, Yuan ;
Geng, Peng ;
Li, Guilin ;
Song, Hongjuan .
BIOCHEMISTRY AND CELL BIOLOGY, 2018, 96 (01) :38-43
123