The Entamoeba histolytica, Arp2/3 Complex Is Recruited to Phagocytic Cups through an Atypical Kinase EhAK1

被引：31

作者：

Babuta, Mrigya ^{[1
]}

Mansuri, M. Shahid ^{[1
]}

Bhattacharya, Sudha ^{[2
]}

Bhattacharya, Alok ^{[1
,3
]}

机构：

[1] Jawaharlal Nehru Univ, Sch Life Sci, New Delhi 110067, India

[2] Jawaharlal Nehru Univ, Sch Environm Sci, New Delhi 110067, India

[3] Shiv Nadar Univ, Dept Life Sci, Sch Nat Sci, Noida, Uttar Pradesh, India

来源：

PLOS PATHOGENS | 2015年 / 11卷 / 12期

关键词：

ACTIN-BINDING PROTEIN; EXPRESSION; PARASITE; ENDOCYTOSIS; SUBUNIT; FAMILY; ROLES; HOST; EHNCABP166; MIGRATION;

D O I：

10.1371/journal.ppat.1005310

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The parasite Entamoeba histolytica is the etiological agent of amoebiasis and phagocytosis plays a key role in virulence of this organism. Signaling pathways involved in activation of cytoskeletal dynamics required for phagocytosis remain to be elucidated. Phagocytosis is initiated with sequential recruitment of EhC2PK, EhCaBP1, EhCaBP3 and an atypical kinase EhAK1 after particle attachment. Here we show that EhARPC1, an essential subunit of the actin branching complex Arp 2/3 is recruited to the phagocytic initiation sites by EhAK1. Imaging, expression knockdown of different molecules and pull down experiments suggest that EhARPC1 interacts with EhAK1 and that it is required during initiation of phagocytosis and phagosome formation. Moreover, recruitment of EhARPC2 at the phagocytosis initiation by EhAK1 is also observed, indicating that the Arp 2/3 complex is recruited. In conclusion, these results suggests a novel mechanism of recruitment of Arp 2/3 complex during phagocytosis in E. histolytica.

引用

页数：27

共 54 条

[1] Efficient uptake of Yersinia pseudotuberculosis via integrin receptors involves a Rac1-Arp 2/3 pathway that bypasses N-WASP function [J].