Evaluation of antifungal efficacy in an optimized animal model of Trichophyton mentagrophytes-dermatophytosis

被引：46
作者：
Ghannoum, MA
Hossain, MA
Long, L
Mohamed, S
Reyes, G
Mukherjee, PK
机构：
[1] Case Western Reserve Univ, Ctr Med Mycol, Cleveland, OH 44106 USA
[2] Univ Hosp Cleveland, Cleveland, OH 44106 USA
来源：
JOURNAL OF CHEMOTHERAPY | 2004年 / 16卷 / 02期
关键词：
antifungal agent; dermatophytosis; efficacy; guinea pig; model;
D O I：
10.1179/joc.2004.16.2.139
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Dermatophytoses are known to cause considerable discomfort, cosmetic problems and financial loss that have been recognized as a significant health concern worldwide. Since currently available antifungal agents have limitations in their efficacy, new agents are being developed. This study was undertaken to optimize an in vivo model of experimental dermatophytosis for evaluation of the efficacy of antifungal compounds. Guinea pigs were infected with different inocula of T. mentagrophytes to establish dermatophytosis. The optimal conditions for dermatophytosis in guinea pigs were found to be an inoculum size of 1 X 107 fungal cells applied on abraded skin. After optimization, animals were treated with oral or topical formulations of terbinafine. The optimized guinea pig model was found to be highly reproducible, and useful in the primary screening and evaluation of the anti-dermatophytic efficacy of topical and oral formulations of antifungal agents.
引用
收藏
页码：139 / 144
页数：6
相关论文
共 16 条
[1]   Open clinical study of the efficacy and safety of terbinafine cream 1% in children with tinea corporis and tinea cruris [J].
Bakos, L ;
Brito, AC ;
Castro, LCM ;
Gontijo, B ;
Lowy, G ;
Reis, CMS ;
Ribeiro, AM ;
Souza, FHC ;
Villar, MD ;
Zaitz, C .
PEDIATRIC INFECTIOUS DISEASE JOURNAL, 1997, 16 (06) :545-548
[2]   Oral terbinafine in the treatment of toenail onychomycosis: North American multicenter trial [J].
Drake, LA ;
Shear, NH ;
Arlette, JP ;
Cloutier, R ;
Danby, FW ;
Elewski, BE ;
GarnisJones, S ;
Giroux, JM ;
Gratton, D ;
Gulliver, W ;
Hull, P ;
Jones, HE ;
Journet, M ;
Krol, AL ;
Leyden, JJ ;
Maddin, SC ;
Ross, JB ;
Savin, RC ;
Scher, RK ;
Sibbald, GR ;
Tawfik, NH ;
Zaias, N ;
Tolpin, M ;
Evans, S ;
Marsolais, C ;
Chin, T ;
Lin, TH ;
Maher, T ;
Birnbaum, JE .
JOURNAL OF THE AMERICAN ACADEMY OF DERMATOLOGY, 1997, 37 (05) :740-745
[3]   LEVELS OF TERBINAFINE IN PLASMA, STRATUM-CORNEUM, DERMIS EPIDERMIS (WITHOUT STRATUM-CORNEUM), SEBUM, HAIR AND NAILS DURING AND AFTER 250 MG TERBINAFINE ORALLY ONCE-DAILY FOR 7 AND 14 DAYS [J].
FAERGEMANN, J ;
ZEHENDER, H ;
MILLERIOUX, L .
CLINICAL AND EXPERIMENTAL DERMATOLOGY, 1994, 19 (02) :121-126
[4]  
FAERGEMANN J, 1993, ACTA DERM-VENEREOL, V73, P305
[5]   New developments in chemotherapy for non-invasive fungal infections [J].
Hossain, MA ;
Ghannoum, MA .
EXPERT OPINION ON INVESTIGATIONAL DRUGS, 2001, 10 (08) :1501-1511
[6]   An evaluation of the in vitro activity of terbinafine [J].
Jessup, CJ ;
Ryder, NS ;
Ghannoum, MA .
MEDICAL MYCOLOGY, 2000, 38 (02) :155-159
[7]  
Jessup CJ, 2000, J CLIN MICROBIOL, V38, P341
[8]  
Niewerth M, 2000, EUR J DERMATOL, V10, P155
[9]   Optimal growth conditions for the determination of the antifungal susceptibility of three species of dermatophytes with the use of a microdilution method [J].
Norris, HA ;
Elewski, BE ;
Ghannoum, MA .
JOURNAL OF THE AMERICAN ACADEMY OF DERMATOLOGY, 1999, 40 (06) :S9-S13
[10]  
PETRANYI G, 1982, SABOURAUDIA, V20, P101
12