Stability of Varicella-Zoster Virus and Herpes Simplex Virus IgG Monoclonal Antibodies

被引：7

作者：

Hart, John ^{[1
]}

Miller, Cheryl ^{[1
]}

Tang, Xiaoling ^{[1
]}

Vafai, Abbas ^{[1
]}

机构：

[1] Natl Ctr Preparedness Detect & Control Infect Dis, Biol Branch, Div Sci Resources, Coordinating Ctr Infect Dis,Ctr Dis Control & Pre, Atlanta, GA 30333 USA

来源：

JOURNAL OF IMMUNOASSAY & IMMUNOCHEMISTRY | 2009年 / 30卷 / 02期

关键词：

HSV; Monoclonal antibody; Stability; VZV; PROTEINS; DIAGNOSTICS; BINDING; ASSAYS;

D O I：

10.1080/15321810902782871

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The stability of 3 monoclonal antibodies was analyzed at various temperatures and freeze/thaw cycles. Two varicella-zoster virus (VZV) IgGs (mAb 4F9 and mAb g62) and 1 herpes simplex virus 1 (HSV-1 mAb 1D4) were selected for these studies. IgGs were either incubated at various temperatures (25C, 37C, 45C, and 60C) for different periods of time (0 to 9 weeks) or processed for several freeze/thaw cycles. The reactivities of mAbs 4F9 (IgG1), g62 (IgG1) and 1D4 (IgG2b) were tested by indirect immunofluorescence assay (IFA). The results indicated that: (1) all three mAbs were stable at 25C and 37C for 9weeks; (2) although the reactivities of mAbs g62 and 1D4 were diminished after 5weeks, mAb 4F9 was stable at 45C for 9weeks; and (3) all 3 IgGs lost reactivity after overnight incubation at 60C. In addition, the results showed that the reactivity of mAbs 4F9, g62 and 1D4 was not diminished after 12 freeze/thaw cycles.

引用

页码：180 / 185

页数：6