The Effect of Concentrated Growth Factor on Rat Bone Marrow Cells In Vitro and on Calvarial Bone Healing In Vivo

被引:47
作者
Takeda, Yukihiro [1 ,2 ]
Katsutoshi, Kokubun [1 ,2 ]
Matsuzaka, Kenichi [1 ,2 ]
Inoue, Takashi [1 ,2 ]
机构
[1] Tokyo Dent Coll, Oral Hlth Sci Ctr, Tokyo 1010061, Japan
[2] Tokyo Dent Coll, Dept Clin Pathophysiol, Tokyo 1010061, Japan
关键词
bone healing; bone marrow; calvarial bone defect; concentrated growth factor; platelet-poor plasma gel; PLATELET-RICH PLASMA; STROMAL CELLS; OSTEOGENIC DIFFERENTIATION; OSTEOBLAST DIFFERENTIATION; STEM-CELLS; FIBRIN; REGENERATION; TGF-BETA-1;
D O I
10.11607/jomi.3995
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
Purpose: Concentrated growth factor (CGF) is an autologous leukocyte- and platelet-rich fibrin biomaterial that has recently been used for bone healing therapy. Previous studies used fibrin membrane or CGF extract without fibrin without soluble factors in vitro. This study investigated the effects of CGF that contained soluble factors on cultured rat bone marrow (RBM) cells in vitro. Furthermore, the effects of CGF implanted in rat calvarial bone defects in vivo were also investigated. Materials and Methods: CGF and platelet-poor plasma (PPP) gel, which both have fibrin networks, were prepared from rat blood. For the in vitro study, CGF and PPP gel were frozen, and thin sections approximately 30 mu m thick were cut and coated onto the surfaces of cell disks. RBM cells were then harvested and cultured on the coated disks in serum-free medium. For the in vivo study, rat calvarial bone defects (3.8 mm in diameter) were created and implanted with CGF, PPP gel, or left unfilled. Results: In vitro, the actin stress fibers of RBM cells cultured on disks coated with CGF or PPP gel tended to extend more than cells cultured on uncoated disks. However, no significant difference was observed in terms of cell morphology between RBM cells cultured on CGF- and PPP gel-coated disks. Cell proliferation and osteoblastic differentiation were significantly higher in cells cultured on the CGF-coated disks than on the PPP gel-coated disks. In vivo, more new bone had formed in defects treated with CGF than in defects treated with PPP gel. Conclusion: In this preliminary study, fibrin and soluble factors in CGF promoted initial cell stretching, proliferation, and osteoblastic differentiation of RBM cells in vitro and bone regeneration in rat calvarial bone defects in vivo.
引用
收藏
页码:1187 / 1196
页数:10
相关论文
共 29 条
  • [1] Fibrin glue as an osteoinductive protein in a mouse model
    Abiraman, S
    Varma, HK
    Umashankar, PR
    John, A
    [J]. BIOMATERIALS, 2002, 23 (14) : 3023 - 3031
  • [2] Autologous platelets as a source of proteins for healing and tissue regeneration
    Anitua, E
    Andia, I
    Ardanza, B
    Nurden, P
    Nurden, AT
    [J]. THROMBOSIS AND HAEMOSTASIS, 2004, 91 (01) : 4 - 15
  • [3] Growth factors and cytokines in wound healing
    Barrientos, Stephan
    Stojadinovic, Olivera
    Golinko, Michael S.
    Brem, Harold
    Tomic-Canic, Marjana
    [J]. WOUND REPAIR AND REGENERATION, 2008, 16 (05) : 585 - 601
  • [4] Boyan B. D., 2003, European Cells & Materials, V6, P22
  • [5] In vitro studies of insulin-like growth factor I and bone
    Conover, CA
    [J]. GROWTH HORMONE & IGF RESEARCH, 2000, 10 : 107 - 110
  • [6] Ehrenfest DMD, 2012, CURR PHARM BIOTECHNO, V13, P1145
  • [7] Classification of platelet concentrates: from pure platelet-rich plasma (P-PRP) to leucocyte- and platelet-rich fibrin (L-PRF)
    Ehrenfest, David M. Dohan
    Rasmusson, Lars
    Albrektsson, Tomas
    [J]. TRENDS IN BIOTECHNOLOGY, 2009, 27 (03) : 158 - 167
  • [8] Eyrich D, 2006, ADV EXP MED BIOL, V585, P379
  • [9] Therapeutic angiogenesis by intramuscular injection of fibrin particles into ischaemic hindlimbs
    Fan, Chun-Ling
    Gao, Ping-Jin
    Gu, Yuan-Jun
    Tang, Xiao-Feng
    Liu, Jian-Jun
    Wei, Jian
    Inoue, Kazutomo
    Zhu, Ding-Liang
    [J]. CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY, 2006, 33 (07): : 617 - 622
  • [10] Hatakeyama I, 2014, TISSUE ENG PT A, V20, P874, DOI [10.1089/ten.TEA.2013.0058, 10.1089/ten.tea.2013.0058]