Enhanced immunomagnetic separation for the detection of norovirus using the polyclonal antibody produced with human norovirus GII.4-like particles

被引：6

作者：

Lee, Kangbum ^{[1
,2
]}

Park, Kwisung ^{[3
]}

Seo, Dong Joo ^{[1
]}

Lee, Min Hwa ^{[1
]}

Jung, Ji-Youn ^{[4
]}

Park, Gyung Jin ^{[5
]}

Yoon, Dasom ^{[6
]}

Park, Ki Hwan ^{[6
]}

Choi, Changsun ^{[1
]}

机构：

[1] Chung Ang Univ, Dept Food & Nutr, Anseong 456756, Gyeonggi, South Korea

[2] Gyeonggi Do Inst Publ Hlth & Environm, Suwon 442701, Gyeonggi, South Korea

[3] Chungcheongnam Do Hlth & Environm Res Inst, Taejon 300801, South Korea

[4] Kongju Natl Univ, Dept Compan & Lab Anim Sci, Kong Ju 314701, Chungnam, South Korea

[5] Kunsan Natl Univ, Dept Food & Nutr, Gunsan 573701, Jeonbuk, South Korea

[6] Chung Ang Univ, Dept Food Sci & Technol, Anseong 456756, Gyeonggi, South Korea

来源：

FOOD SCIENCE AND BIOTECHNOLOGY | 2014年 / 23卷 / 05期

关键词：

norovirus; immunomagnetic separation; virus-like particles (VLPs); polyclonal antibody; concentration; VIRUS-LIKE PARTICLES; REVERSE TRANSCRIPTION-PCR; NORWALK VIRUS; HUMAN CALICIVIRUSES; RAPID DETECTION; SAMPLES; GASTROENTERITIS; ASSAYS; DIAGNOSIS; CHILDREN;

D O I：

10.1007/s10068-014-0213-2

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Human norovirus (NoV) is the most common cause of foodborne viral gastroenteritis worldwide. This study was aimed to develop the enhanced immunomagnetic separation (IMS) for effectively concentrating and detecting human noroviruses in food matrix. Virus-like particles (VLPs) were made by integrating NoV GII.4 capsid gene into baculovirus vector. In order to increase the sensitivity and specificity of immunomagnetic complex, polyclonal rabbit antibody against NoV GII.4 capsid was produced and used for producing immunomagnetic beads. IMS, polyethylene glycol precipitation, and ultrafiltration were compared to concentrate NoV spiked in vegetables. IMS was the most efficient method for concentrating NoV. Therefore, IMS developed in this study is the most effective method to concentrate and detect NoV contaminated in produce.

引用

页码：1569 / 1576

页数：8

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