Effects of gonadotropin-releasing hormone on growth hormone secretion and gene expression in common carp pituitary

被引:39
作者
Li, WS
Lin, HR [1 ]
Wong, AOL
机构
[1] Zhongshan Univ, Sch Life Sci, Guangzhou 510275, Peoples R China
[2] Univ Hong Kong, Dept Zool, Hong Kong, Hong Kong, Peoples R China
来源
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY | 2002年 / 132卷 / 02期
关键词
gonadotropin-releasing hormone; growth hormone; growth hormone mRNA; analog of gonadotropin-releasing hormone; radioimmunoassay; ribonuclease protection assay; common carp; pituitary;
D O I
10.1016/S1096-4959(02)00039-8
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Using radioimmuno- and ribonuclease protection assays, we examined the effects of gonadotropin-releasing hormone and its analogs on the growth hormone mRNA level and growth hormone secretion in common carp (Cyprinus carpio) pituitary fragments with static incubation. After a 24 h treatment, sGnRH ([Trp(7) Leu(8)]-LHRH) and sGnRH-A ([D-Arg (6), Pro(9)]-LHRH) (0.1 nM-1 muM) elevated the GH mRNA level and stimulated the GH secretion in a dose-dependent manner, with a higher potency for sGnRH-A. In a time-course experiment, the function of sGnRH and sGnRH-A (10 nM) on GH secretion was observed after 6 h incubation, while no action on the GH mRNA level were noted until 12 h after treatment. Comparing mammalian GnRH, avian GnRH and piscine GnRH, sGnRH and sGnRH-A showed the highest potency in increasing GH mRNA level and GH-release, followed by cGnRH-II ([His(5),Tyr(8)]-LHRH), and finally LHRH and LHRH-A([D-Trp(6), Pro(9)]-LHRH). These findings, taken together, suggest that GnRH not only can influence GH release, but also play a role in the regulation of GH synthesis. (C) 2002 Elsevier Science Inc. All rights reserved.
引用
收藏
页码:335 / 341
页数:7
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