Construction of vectors and a genomic library for use with his3-deficient strains of Schizosaccharomyces pombe

被引：41

作者：

Ohi, R ^{[1
]}

Feoktistova, A ^{[1
]}

Gould, KL ^{[1
]}

机构：

[1] VANDERBILT UNIV, SCH MED, HOWARD HUGHES MED INST, DEPT CELL BIOL, NASHVILLE, TN 37232 USA

来源：

GENE | 1996年 / 174卷 / 02期

关键词：

fission yeast; plasmids; cloning; gene disruption; recombinant DNA;

D O I：

10.1016/0378-1119(96)00085-6

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The construction of vectors for use in Schizosaccharomyces pombe using the his3(+) gene as a selectable marker is described. In addition, we report the construction of a genomic library in a his3(+)-containing shuttle vector to facilitate the cloning of genes by complementation of mutant function in strains defective for His3 activity.

引用

页码：315 / 318

页数：4

共 7 条

[1] VERSATILE SHUTTLE VECTORS AND GENOMIC LIBRARIES FOR USE WITH SCHIZOSACCHAROMYCES-POMBE
BARBET, N
MURIEL, WJ
CARR, AM
[J]. GENE, 1992, 114 (01) : 59 - 66
[2] MOLECULAR-CLONING AND CHARACTERIZATION OF THE SCHIZOSACCHAROMYCES-POMBE HIS3 GENE FOR USE AS A SELECTABLE MARKER
BURKE, JD
GOULD, KL
[J]. MOLECULAR AND GENERAL GENETICS, 1994, 242 (02): : 169 - 176
[3] Jones E.W., 1982, MOL BIOL YEAST SACCH, P181
[4] MORENO S, 1991, METHOD ENZYMOL, V194, P795
[5] HIGH-EFFICIENCY TRANSFORMATION OF SCHIZOSACCHAROMYCES-POMBE BY ELECTROPORATION
PRENTICE, HL
[J]. NUCLEIC ACIDS RESEARCH, 1992, 20 (03) : 621 - 621
[6] NEGATIVE REGULATION OF MITOSIS BY WEE1+, A GENE ENCODING A PROTEIN-KINASE HOMOLOG
RUSSELL, P
NURSE, P
[J]. CELL, 1987, 49 (04) : 559 - 567
[7] RUSSELL P, 1989, MOL BIOL FISSION YEA, P244

← 1 →