Aberrant histone modification in CD19+ B cells of patients with chronic lymphocytic leukemia

被引：5

作者：

Zhou, Keshu ^{[1
]}

Zhang, Qing ^{[1
]}

Liu, Yanyan ^{[1
]}

Xiong, Yuanyuan ^{[1
]}

Wu, Shengsheng ^{[1
]}

Yang, Jingke ^{[1
]}

Zhou, Hu ^{[1
]}

Liu, Xinjian ^{[1
]}

Wei, Xudong ^{[1
]}

Song, Yongping ^{[1
]}

机构：

[1] Zhengzhou Univ, Henan Canc Hosp, Dept Hematol, Affiliated Canc Hosp, 127 Dongmin Rd, Zhengzhou 450008, Henan, Peoples R China

来源：

ONCOTARGETS AND THERAPY | 2017年 / 10卷

基金：

中国国家自然科学基金;

关键词：

histone methylation; histone acetylation; SIRT1; EZH2; CLL; ZESTE HOMOLOG 2; EZH2; CANCER; OVEREXPRESSION; METHYLTRANSFERASE; DEACETYLASE; ENHANCER; DISEASE; SIRT1;

D O I：

10.2147/OTT.S121301

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The aim of this study was to detect the alterations in histone methylation and acetylation in patients with chronic lymphocytic leukemia (CLL). Global histone H3/H4 acetylation and H3K4/H3K9 methylation were detected by the EpiQuik (TM) global histone H3/H4 acetylation and H3K4/H3K9 methylation assay kits. The mRNA expression of selected chromatin modifier genes was measured by real-time polymerase chain reaction (RT-PCR). Our results found that the global histone H3/H4 hypoacetylation in the CD19(+) B cells of patients with CLL (P=0.028 and P=0.03, respectively) and the global histone H3K9 methylation in patients with CLL were significantly increased compared with controls (P=0.02), while there was no significant difference in the global histone H3K4 methylation between the two groups. The level of SIRT1 and EZH2 mRNA expression was upregulated in patients with CLL (P=0.03 and P=0.02, respectively), which increased significantly with progression from Binet stage A to stage C (P=0.015 and P=0.01, respectively) and Rai good to high risk stage (P=0.007 and P=0.008, respectively). The level of HDAC1 and HDAC7 mRNA expression was significantly increased (P=0.02 and P=0.008, respectively) and HDAC2 and P300 mRNA expression was reduced in patients with CLL (P=0.002 and P=0.001, respectively). In conclusion, it is observed that the aberrant histone modification plays an important role in the pathogenesis of CLL.

引用

页码：1173 / 1179

页数：7

共 19 条

[1] Depsipeptide (FR901228) induces histone acetylation and inhibition of histone deacetylase in chronic lymphocytic leukemia cells concurrent with activation of caspase 8-mediated apoptosis and down-regulation of c-FLIP protein [J].