Isolation and characterization of novel microsatellite loci in Nibea albiflora

被引：7
作者：
Xu, D. D. [1 ]
Lou, B. [1 ]
Li, S. L. [1 ]
Zhang, Y. R. [1 ]
Xin, J. [1 ]
Zhan, W. [1 ]
机构：
[1] Marine Fishery Inst Zhejiang Prov, Key Lab Mariculture & Enhancement Zhejiang Prov, Zhoushan, Zhejiang, Peoples R China
来源：
GENETICS AND MOLECULAR RESEARCH | 2013年 / 12卷 / 04期
关键词：
Nibea albiflora; Microsatellite; Polymorphism; GENETICS;
D O I：
10.4238/2013.December.4.1
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Eleven novel microsatellite loci were isolated from a (CA)(10)-enriched genomic DNA library of Nibea albiflora. The characteristics of these microsatellites were determined in a sample of 48 N. albiflora individuals. The number of alleles at the 11 microsatellite loci ranged from 5 to 25, with an average of 13.5 per locus. The observed and expected heterozygosities varied from 0.583 to 0.917 and from 0.568 to 0.964, respectively. Eight of the 11 microsatellite loci conformed to the Hardy-Weinberg equilibrium. No significant linkage disequilibrium was found among all 11 loci. These polymorphic microsatellites will be useful for population genetic analyses of N. albiflora.
引用
收藏
页码：6156 / 6159
页数：4
相关论文
共 13 条
  • [1] Arlequin (version 3.0): An integrated software package for population genetics data analysis
    Excoffier, Laurent
    Laval, Guillaume
    Schneider, Stefan
    [J]. EVOLUTIONARY BIOINFORMATICS, 2005, 1 : 47 - 50
  • [2] Microsatellite enrichment in organisms with large genomes (Allium cepa L.)
    Fischer, D
    Bachmann, K
    [J]. BIOTECHNIQUES, 1998, 24 (05) : 796 - +
  • [3] Genetic population structure of Nibea albiflora in yellow sea and east china sea
    Han, Zhi Qiang
    Gao, Tian Xiang
    Yanagimoto, Takashi
    Sakurai, Yasunori
    [J]. FISHERIES SCIENCE, 2008, 74 (03) : 544 - 552
  • [4] Han ZQ, 2006, J FISH CHINA, V5, P646
  • [5] DNA marker technologies and their applications in aquaculture genetics
    Liu, ZJ
    Cordes, JF
    [J]. AQUACULTURE, 2004, 238 (1-4) : 1 - 37
  • [6] Isolation and characterization of 16 polymorphic microsatellite markers from Nibea albiflora
    Ma, Chunyan
    Ma, Hongyu
    Ma, Lingbo
    Jiang, Keji
    Cui, Haiyu
    Ma, Qunqun
    [J]. FISHERIES SCIENCE, 2011, 77 (05) : 707 - 711
  • [7] GENEPOP (VERSION-1.2) - POPULATION-GENETICS SOFTWARE FOR EXACT TESTS AND ECUMENICISM
    RAYMOND, M
    ROUSSET, F
    [J]. JOURNAL OF HEREDITY, 1995, 86 (03) : 248 - 249
  • [8] Rozen S, 2000, Methods Mol Biol, V132, P365
  • [9] Takita T., 1974, Bulletin Fac Fish Nagasaki Univ, VNo. 38, P1
  • [10] Isolation and characterization of polymorphic microsatellite loci from a dinucleotide-enriched genomic library of spotted maigre (Nibea albiflora)
    Xing, Shichao
    Shao, Changwei
    Liao, Xiaolin
    Tian, Yongsheng
    Chen, Songlin
    [J]. CONSERVATION GENETICS, 2009, 10 (03) : 789 - 791
12