Enhanced Production of Poly (γ-glutamic acid) from Bacillus licheniformis NCIM 2324 by Using Metabolic Precursors

被引：55
|
作者：
Bajaj, Ishwar B. [1 ]
Singhal, Rekha S. [1 ]
机构：
[1] Univ Mumbai, Food Engn & Technol Dept, Inst Chem Technol, Bombay 400019, Maharashtra, India
来源：
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 2009年 / 159卷 / 01期
关键词：
Poly (gamma-glutamic acid); Fermentation; Bacillus licheniformis; Precursors; alpha-Ketoglutaric acid; POLY(GAMMA-GLUTAMIC ACID); POLY(GAMMA-D-GLUTAMYL) CAPSULE; SUBTILIS IF03335; AMMONIUM-SULFATE; CITRIC-ACID; BIOSYNTHESIS; MICROORGANISMS; OPTIMIZATION; CHEMISTRY;
D O I：
10.1007/s12010-008-8427-5
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The aim of the present work was to study the effect of addition of different amino acids and tricarboxylic acid cycle intermediates as metabolic precursors on the production of poly (gamma-glutamic acid) (PGA) by Bacillus licheniformis NCIM 2324. A maximum yield of 35.75 g/l was obtained with the medium supplemented with 0.5 mM l-glutamine and 10 mM alpha-ketoglutaric acid as compared to 26.12 g/l PGA achieved with the control in the absence of metabolic precursors. Addition of precursors also enhanced the utilization of l-glutamic acid during fermentation.
引用
收藏
页码：133 / 141
页数：9
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