Evidence that TRPM7 is required for breast cancer cell proliferation

被引:205
作者
Guilbert, Arnaud [1 ]
Gautier, Mathieu [1 ]
Dhennin-Duthille, Isabelle [1 ]
Haren, Nathalie [1 ]
Sevestre, Henri [1 ,2 ]
Ouadid-Ahidouch, Halima [1 ]
机构
[1] Ctr Hosp Univ Nord, Lab Physiol Cellulaire & Mol, JE Canaux Ion Canc Sein 2530, Fac Sci, Amiens, France
[2] Ctr Hosp Univ Nord, Serv Anat Pathol, Amiens, France
来源
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY | 2009年 / 297卷 / 03期
关键词
transient receptor potential; MAMMARY EPITHELIAL-CELLS; ANOXIC NEURONAL DEATH; CA2+ ENTRY; SIGNAL-TRANSDUCTION; PROSTATE-CANCER; CALCIUM-ENTRY; MUSCLE-FIBERS; CHANNELS; KINASE; INHIBITION;
D O I
10.1152/ajpcell.00624.2008
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Guilbert A, Gautier M, Dhennin-Duthille I, Haren N, Sevestre H, Ouadid-Ahidouch H. Evidence that TRPM7 is required for breast cancer cell proliferation. Am J Physiol Cell Physiol 297: C493-C502, 2009. First published June 10, 2009; doi:10.1152/ajpcell.00624.2008.-Because transient receptor potential (TRP) channels have been implicated in tumor progression, we have investigated the potential role of TRPM7 channel in breast cancer cell proliferation. Under whole cell patch clamp, a Mg2+-inhibited cationic (MIC) current was observed in MCF-7 cells. This current was characterized by an inward current and a strong outward rectifying current that were both inhibited in a concentration-dependent manner by the presence of intracellular Mg2+ or Mg2+-ATP. The inward current was reduced by La3+, and the outward current was sensitive to 2-aminoethoxydiphenyl borate (2-APB), spermine, La3+, and flufenamic acid. Importantly, a similar MIC current was also recorded in the primary culture of human breast cancerous epithelial cells (hBCE). Moreover, TRPM7 transcripts were found in both hBCE and MCF-7 cells. In MCF-7 cells, the MIC current was inhibited by TRPM7 small interfering RNA. Interestingly, we found that cell proliferation and intracellular Ca2+ concentration were also reduced by TRPM7 silencing in MCF-7 cells. TRPM7 channels were also found in both human breast cancer and healthy tissues. Importantly, TRPM7 channel was overexpressed in grade III breast cancer samples associated with important Ki67 or tumor size. Our findings strongly suggest that TRPM7 is involved in the proliferative potentiality of breast cancer cells, probably by regulating Ca2+ influx.
引用
收藏
页码:C493 / C502
页数:10
相关论文
共 41 条
  • [1] A key role for TRPM7 channels in anoxic neuronal death
    Aarts, M
    Iihara, K
    Wei, WL
    Xiong, ZG
    Arundine, M
    Cerwinski, W
    MacDonald, JF
    Tymianski, M
    [J]. CELL, 2003, 115 (07) : 863 - 877
  • [2] Importance of melastatin-like transient receptor potential 7 and cations (magnesium, calcium) in human osteoblast-like cell proliferation
    Abed, E.
    Moreau, R.
    [J]. CELL PROLIFERATION, 2007, 40 (06) : 849 - 865
  • [3] Emerging roles of TRPM6/TRPM7 channel kinase signal transduction complexes
    Chubanov, V
    Schnitzler, MMY
    Wäring, J
    Plank, A
    Gudermann, T
    [J]. NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY, 2005, 371 (04) : 334 - 341
  • [4] TRPM7, a novel regulator of actomyosin contractility and cell adhesion
    Clark, K
    Langeslag, M
    van Leeuwen, B
    Ran, L
    Ryazanov, AG
    Figdor, CG
    Moolenaar, WH
    Jalink, K
    van Leeuwen, FN
    [J]. EMBO JOURNAL, 2006, 25 (02) : 290 - 301
  • [5] TRPM7 regulates myosin IIA filament stability and protein localization by heavy chain phosphorylation
    Clark, Kristopher
    Middelbeek, Jeroen
    Lasonder, Edwin
    Dulyaninova, Natalya G.
    Morrice, Nick A.
    Ryazanov, Alexey G.
    Bresnick, Anne R.
    Figdor, Carl G.
    van Leeuwen, Frank N.
    [J]. JOURNAL OF MOLECULAR BIOLOGY, 2008, 378 (04) : 790 - 803
  • [6] Calcium-sensing receptor stimulation induces nonselective cation channel activation in breast cancer cells
    El Hiani, Yassine
    Ahidouch, Ahmed
    Roudbaraki, Morad
    Guenin, Stephanie
    Brule, Gerard
    Ouadid-Ahidouch, Halima
    [J]. JOURNAL OF MEMBRANE BIOLOGY, 2006, 211 (02) : 127 - 137
  • [7] ETHIER SP, 1993, CANCER RES, V53, P627
  • [8] Expression of TRPC6 channels in human epithelial breast cancer cells
    Guilbert, Arnaud
    Dhennin-Duthille, Isabelle
    Hiani, Yassine E. L.
    Haren, Nathalie
    Khorsi, Hafida
    Sevestre, Henri
    Ahidouch, Ahmed
    Ouadid-Ahidouch, Halima
    [J]. BMC CANCER, 2008, 8 (1)
  • [9] Involvement of TRPM7 in cell growth as a spontaneously activated Ca2+ entry pathway in human retinoblastoma cells
    Hanano, T
    Hara, Y
    Shi, J
    Morita, H
    Umebayashi, C
    Mori, E
    Sumimoto, H
    Ito, Y
    Mori, Y
    Inoue, R
    [J]. JOURNAL OF PHARMACOLOGICAL SCIENCES, 2004, 95 (04) : 403 - 419
  • [10] Dissociation of the store-operated calcium current/CRAC and the Mg-nucleotide-regulated metal ion current MagNuM
    Hermosura, MC
    Monteilh-Zoller, MK
    Scharenberg, AM
    Penner, R
    Fleig, A
    [J]. JOURNAL OF PHYSIOLOGY-LONDON, 2002, 539 (02): : 445 - 458