Hypoxia promotes the invasion and metastasis of laryngeal cancer cells via EMT

被引：71
作者：
Zuo, Jianhong [1 ,2 ]
Wen, Juan [1 ]
Lei, Mingsheng [3 ]
Wen, Meiling [2 ]
Li, Sai [1 ,2 ]
Lv, Xiu [1 ,2 ]
Luo, Zhaoyang [1 ]
Wen, Gebo [1 ]
机构：
[1] Univ South China, Sch Med, Hengyang 421001, Hunan, Peoples R China
[2] Univ South China, Nanhua Hosp, Hengyang 421000, Hunan, Peoples R China
[3] Zhangjiajie City Hosp, Dept Resp & Crit Care Med, Zhangjiajie 427000, Hunan, Peoples R China
来源：
MEDICAL ONCOLOGY | 2016年 / 33卷 / 02期
关键词：
Laryngeal carcinoma; E-cadherin; Vimentin; Glucose transporter-1; Epithelial-mesenchymal transition; EPITHELIAL-MESENCHYMAL TRANSITION; INDUCIBLE FACTOR-1-ALPHA; N-CADHERIN; GLUCOSE TRANSPORTERS; PROGNOSTIC VALUE; DOWN-REGULATION; POOR-PROGNOSIS; EXPRESSION; CARCINOMA; GLUCOSE-TRANSPORTER-1;
D O I：
10.1007/s12032-015-0716-6
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
The purpose of this study is to explore the role of hypoxia on the invasion and metastasis of laryngeal carcinoma. The invasion and migration ability of laryngeal cancer SCC10A cell was detected by transwell assay. Western blot was applied to analyze the expression of EMT-related proteins. Fifty-seven samples from postoperative patients with laryngeal cancer were collected to study. Immunohistochemistry was used to examine the expression of GLUT-1 and EMT-related proteins (Vim, E-cad, N-cad) in normal laryngeal squamous epithelial tissue, laryngeal cancer adjacent tissues and laryngeal squamous cell carcinoma tissues. Hypoxia promoted laryngeal cancer cell invasion and migration. Hypoxia also enhanced the expression of GLUT-1, vimentin and N-cad, which exist statistically significant correlation with the clinical staging and lymph node meta-stases (P< 0.05). The expression of GLUT-1 is positively correlated with Vim and N-cad expression in laryngeal squamous cell carcinoma tissues, but negatively correlated with E-cad expression. The patient survival rate with the positive expression of GLUT-1, Vim and N-cad becomes much shorter compared with those with negative expression of GLUT-1, Vim and N-cad (P< 0.05). Hypoxia promoted laryngeal cancer cell invasion and migration via EMT.
引用
收藏
页码：1 / 9
页数：9
相关论文
共 50 条
[1]   GLUT-1 Expression in Cutaneous Basal and Squamous Cell Carcinomas [J].
Abdou, Asmaa Gaber ;
Eldien, Marwa Mohammad Serag ;
Elsakka, Daliah .
INTERNATIONAL JOURNAL OF SURGICAL PATHOLOGY, 2015, 23 (06) :447-453
[2]   Glucose transporter-1 expression in CD133+ laryngeal carcinoma Hep-2 cells [J].
Chen, Xiao-Hong ;
Bao, Yang-Yang ;
Zhou, Shui-Hong ;
Wang, Qin-Ying ;
Wei, Yan ;
Fan, Jun .
MOLECULAR MEDICINE REPORTS, 2013, 8 (06) :1695-1700
[3]   Overexpression of MMP-9 and HIF-1α in Breast Cancer Cells under Hypoxic Conditions [J].
Choi, Jae Young ;
Jang, Yeon Soo ;
Min, Sun Young ;
Song, Jeong Yoon .
JOURNAL OF BREAST CANCER, 2011, 14 (02) :88-95
[4]   A novel HIF-1α-integrin-linked kinase regulatory loop that facilitates hypoxia-induced HIF-1α expression and epithelial-mesenchymal transition in cancer cells [J].
Chou, Chih-Chien ;
Chuang, Hsaio-Ching ;
Salunke, Santosh B. ;
Kulp, Samuel K. ;
Chen, Ching-Shih .
ONCOTARGET, 2015, 6 (10) :8271-8285
[5]   N-cadherin regulates mammary tumor cell migration through Akt3 suppression [J].
Chung, S. ;
Yao, J. ;
Suyama, K. ;
Bajaj, S. ;
Qian, X. ;
Loudig, O. D. ;
Eugenin, E. A. ;
Phillips, G. R. ;
Hazan, R. B. .
ONCOGENE, 2013, 32 (04) :422-430
[6]   Upregulation of glucose metabolism by NF-κB2/p52 mediates enzalutamide resistance in castration-resistant prostate cancer cells [J].
Cui, Yuanyuan ;
Nadiminty, Nagalakshmi ;
Liu, Chengfei ;
Lou, Wei ;
Schwartz, Chad T. ;
Gao, Allen C. .
ENDOCRINE-RELATED CANCER, 2014, 21 (03) :435-442
[7]   FDG uptake in primary squamous cell carcinoma of the head and neck The relationship between overexpression of glucose transporters and hexokinases, tumour proliferation and apoptosis [J].
Deron, P. ;
Vangestel, C. ;
Goethals, I. ;
De Potter, A. ;
Peeters, M. ;
Vermeersch, H. ;
Van de Wiele, C. .
NUKLEARMEDIZIN-NUCLEAR MEDICINE, 2011, 50 (01) :15-21
[8]  
Deron P, 2011, HISTOL HISTOPATHOL, V26, P1165, DOI 10.14670/HH-26.1165
[9]   Expression of Glut-1 is a prognostic marker for oral squamous cell carcinoma patients [J].
Eckert, A. W. ;
Lautner, M. H. W. ;
Taubert, H. ;
Schubert, J. ;
Bilkenroth, U. .
ONCOLOGY REPORTS, 2008, 20 (06) :1381-1385
[10]   Coexpression of hypoxia-inducible factor-1α and glucose transporter-1 is associated with poor prognosis in oral squamous cell carcinoma patients [J].
Eckert, Alexander W. ;
Lautner, Matthias H. W. ;
Schuetze, Andreas ;
Taubert, Helge ;
Schubert, Johannes ;
Bilkenroth, Udo .
HISTOPATHOLOGY, 2011, 58 (07) :1136-1147
12345