Purification and characterization of carboxymethylcellulase from Bacillus pumilus EWBCM1 isolated from earthworm gut (Eudrilus eugeniae)

The present work describes purification and characterization of an extracellular cellulase from a newly isolated cellulolytic bacterial strain Bacillus pumilus EWBCM1. The candidate strain was tested for its abilities to hydrolyze the structural polysaccharides through the depolymerising activities of carboxymethyl-cellulase. The purification of cellulase was carried out by ammonium sulphate precipitation, DEAE cellulose and sephadex G-100. Purified cellulase was found to be 47.56 fold along with 1.46 U/mg of protein and the molecular weight of cellulase was 47 kDa through SDS-PAGE electrophoresis. The optimum activity of cellulase was registered at pH 10 at 50 degrees C. In the present study, metal ions inferred the addition of 5 mM CaCl2 (128%) was recorded good activity than the other tested metal ions. Maximum activity of purified cellulase was recorded in 3% of NaCl and 5% of surfactants medium. The bioactive of purified cellulase was carried out with various commercial detergent and antimicrobial activity. (C) 2020 Published by Elsevier B.V. on behalf of King Saud University.