Method with high-throughput screening potential for antioxidative substances using Escherichia coli biosensor katG′::lux

被引：10

作者：

Tienaho, Jenni ^{[1
,2
]}

Sarjala, Tytti ^{[2
]}

Franzen, Robert ^{[1
]}

Karp, Matti ^{[1
]}

机构：

[1] Tampere Univ Technol, Dept Chem & Bioengn, FI-33101 Tampere, Finland

[2] Nat Resources Inst Finland Luke, Parkano Res Unit, FI-39700 Parkano, Finland

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2015年 / 118卷

关键词：

Antioxidative activity; Bacterial biosensor; Bioscreening; Microplate technique; ASSAY;

D O I：

10.1016/j.mimet.2015.08.018

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A new method is described for the rapid real-time screening of antioxidative properties using a recombinant Escherichia coli DPD2511 biosensor. This microplate technique, without time-consuming pre-incubations and handling, has potential for a high-throughput search of bioactive compounds. Special emphasis was given to obtaining highly reliable and repeatable results. (C) 2015 Elsevier B.V. All rights reserved.

引用

页码：78 / 80

页数：3

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