Perylenebisimide-linked nitroxide for the detection of hydroxyl radicals

被引:33
|
作者
Maki, Tomoharu [1 ]
Soh, Nobuaki [1 ]
Fukaminato, Tuyoshi [1 ]
Nakajima, Hizuru [1 ]
Nakano, Koji [1 ]
Imato, Toshihiko [1 ]
机构
[1] Kyushu Univ, Grad Sch Engn, Dept Appl Chem, Nishi Ku, Fukuoka 8190395, Japan
关键词
Hydroxyl radical; Perylene; Nitroxide; Fluorescent; Fluorescent probe; FLUORESCENT-PROBE; FLUOROPHORE; HYDROPEROXIDES; SUPEROXIDE; COUMARIN; MOLECULE; CELLS; ASSAY;
D O I
10.1016/j.aca.2009.02.044
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A new type of fluorophore-linked nitroxide for the detection of hydroxyl radical ((OH)-O-center dot) was synthesized using perylene-3,4,9,10-tetracarboxyl bisimide and 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO). This new fluorescent probe, indicated here as Spy-OH (swallow-tailed perylene derivative for detecting (OH)-O-center dot) has its natural fluorescence effectively reduced due to the quenching caused by the presence of the nitroxide radical in its structure. In the presence of (OH)-O-center dot, in media containing DMSO, a conspicuous increase in the fluorescence intensity was observed. No significant change in the fluorescence intensity was observed upon the addition of other several reactive oxygen species (ROS) except for (OH)-O-center dot. Spy-OH maxima excitation and emission wavelengths lie in the visible region (520/530 nm), therefore, this fluorescent probe is expected to be useful for a simple and selective detection of (OH)-O-center dot, especially for biosamples. (C) 2009 Elsevier B.V. All rights reserved.
引用
收藏
页码:78 / 82
页数:5
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