Effect of cold atmospheric pressure plasma on DNA integrity in patients with asthenospermia

In this paper, we focused on the possible biomedical applications of the atmospheric pressure plasma, where we used plasma ignited in Argon (Ar) gas flow to study the effects of cold plasma on DNA integrity. Atmospheric pressure plasma, by needle, contains almost of equal amounts of positive and negative charges and has much potential for medical applications. A prospective study carried on 60 samples of human semen; each sample divided into 2 parts, before and after exposure to plasma, and then study the DNA integrity in both parts. Sperm DNA integrity was determined using a modified alkaline single cell gel electrophoresis (comet assay) and acridine orange test (AOT). The results showed that atmospheric pressure plasma give a significant (P<0.05) decreasing in DNA damage for prepared sperm. Plasma for sperm preparation, decreases DNA damage.