A New Lipid Anchor for Sparsely Tethered Bilayer Lipid Membranes

被引：117

作者：

Heinrich, Frank ^{[1
,3
]}

Ng, Tiffany ^{[4
]}

Vanderah, David J. ^{[2
]}

Shekhar, Prabhanshu ^{[3
]}

Mihailescu, Mihaela ^{[1
,5
]}

Nanda, Hirsh ^{[1
]}

Losche, Mathias ^{[1
,3
]}

机构：

[1] Natl Inst Stand & Technol, Ctr Neutron Res, Gaithersburg, MD 20899 USA

[2] NIST, Chem Sci & Technol Lab, Gaithersburg, MD 20899 USA

[3] Carnegie Mellon Univ, Dept Phys, Pittsburgh, PA 15213 USA

[4] Johns Hopkins Univ, Dept Biomed Engn, Baltimore, MD 21218 USA

[5] Univ Calif Irvine, Sch Med, Dept Physiol & Biophys, Irvine, CA 92697 USA

来源：

LANGMUIR | 2009年 / 25卷 / 07期

关键词：

SELF-ASSEMBLED MONOLAYERS; 1-THIAHEXA(ETHYLENE OXIDE); POLY(ETHYLENE OXIDE); SUPPORTED MEMBRANES; NEUTRON REFLECTION; PROTEIN ADSORPTION; SURFACE; PHOSPHATIDYLCHOLINE; INTERFACE; INSERTION;

D O I：

10.1021/la8033275

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Mixed self-assembled monolayers (SAMs) of beta-mercaptoethanol and the new synthetic lipid 1,2-dipalmityl-3[omega-mercaptonona(ethylene oxide)] glycerol (FC16) were investigated for their ability to form sparsely tethered bilayer lipid membranes (stBLMs) completed with various phospholipids. We investigated the structural and functional properties of FC16-based stBLMs and compared these to stBLMs prepared using a previously characterized synthetic lipid, 1,2-dimyristyl-3-[omega-mercaptohexa(ethylene oxide)] glycerol (WC14). FC16-based stBLMs show increased resistivity to ion transfer and an increase in the submembrane space of similar to 0.5 rim. Importantly, FC16-based stBLMs formed well-defined, complete bilayers with charged phospholipids such as 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoglycerol (POPG). In these, POPG incorporates into the outer monolayer leaflet in the same ratio as in the immersion solution but is excluded from the inner leaflet. In all cases that we have investigated thus far, the area densities of the lipids within the bilayers were on average close to those in free bilayer membranes. For charged phospholipids, FC16 appears to provide a distinct advantage over WC14 for the formation of well-defined stBLMs.

引用

页码：4219 / 4229

页数：11

共 37 条

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