Flavobacterium eburneum sp nov., isolated from reclaimed saline land soil

A novel Gram-stain-negative, motile by gliding, and aerobic bacterial strain, designated SA31T, was isolated from reclaimed saline land soil near a lake in Taean-gun, South Korea. Cells of the isolate formed ivory-coloured colonies. Growth occurred at 10-35 degrees C (optimum 25-30 degrees C), pH 6.0-9.0 (optimum pH 7.0-7.5), and 0-2.0% (w/v) NaCl (optimum 0%). Based on similarities of 16S rRNA gene sequences, strain SA31(T) was mostly affiliated with the genus Flavobacterium, exhibiting the highest sequence similarities with Flavobacterium palustre S44(T) (96.0%), Flavobacterium glycines Gm-149(T) (95.9%), Flavobacterium defluvii EMB117(T) (95.7%) and Flavobacterium daejeonense GH1- 10(T) (95.6%). Phylogenetic analysis based on 16S rRNA gene sequences also indicated that strain SA31(T) was clustered with Flavobacterium daejeonense GH1- 10(T) and Flavobacterium glycines Gm- 149(T). The predominant fatty acids (>7% of total) of strain SA31(T) were iso-C-15:0, summed feature 3 (C-16:1 omega 7c and/or C-16:1 omega 6c), iso-C-17:0 3-OH and iso-C-15:0 3-OH. The major polar lipids of the isolate comprised phosphatidylethanolamine, one unknown aminolipid, one unknown glycolipid, one unknown aminophospholipid and three unknown lipids. The major respiratory quinone was MK-6. The genomic DNA G+C content of strain SA31(T) was 33.5 mol%. On the basis of phenotypic, chemotaxonomic and phylogenetic analyses, strain SA31(T) represents a novel species the genus Flavobacterium, for which the name Flavobacterium eburneum sp. nov. is proposed. The type strain is SA31(T) (=KACC 18743(T) =JCM 31221T