A magnetic colloid system for isolation of rare cells from blood for fish analysis

被引：1
|
作者：
Martin, B
Bruce, J
Smith, N
Liu, D
Durrant, L
机构：
[1] CITY HOSP NOTTINGHAM,DEPT OBSTET & GYNAECOL,NOTTINGHAM NG5 1PB,ENGLAND
[2] CITY HOSP NOTTINGHAM,DEPT CYTOGENET,NOTTINGHAM NG5 1PB,ENGLAND
来源：
PRENATAL DIAGNOSIS | 1997年 / 17卷 / 11期
关键词：
magnetic cell sorting; FISH; trophoblast; non-invasive; NRBC;
D O I：
10.1002/(SICI)1097-0223(199711)17:11<1059::AID-PD196>3.0.CO;2-9
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
To improve cell recovery of trophoblast and nucleated red blood cells from maternal blood for diagnosis of chromosomal abnormalities, we have investigated the use of a magnetic sorting system that utilizes a ferrofluid. The main advantage of this system is that the beads used are small enough to allow visualization of chromosome-specific sequences by fluorescence in situ hybridization (FISH). The ferrofluid was validated using MAb340, a trophoblast-specific antibody, and anti-CD71, used to sort for nucleated red blood cells. Antigen-positive cells could be efficiently sorted from a 1000-fold excess of antigen-negative cells and easily stained by FISH. We are currently evaluating its use on maternal blood samples. (C) 1997 by John Wiley & Sons, Ltd.
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收藏
页码：1059 / 1066
页数：8
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