Prevalence and quantification of Listeria monocytogenes in beef offal at retail level in Selangor, Malaysia

被引:12
作者
Kuan, Chee Hao [1 ]
Wong, Woan Chwen [1 ]
Pui, Chai Fung [1 ]
Mahyudin, Nor Ainy [1 ]
Tang, John Yew Huat [2 ]
Nishibuchi, Mitsuaki [3 ]
Radu, Son [1 ]
机构
[1] Univ Putra Malaysia, Fac Food Sci & Technol, Food Safety Res Ctr, Serdang 43400, Darul Ehsan, Malaysia
[2] Univ Sultan Zainal Abidin, Fac Food Technol, Terengganu Darul, Iman, Malaysia
[3] Kyoto Univ, Ctr Southeast Asian Studies, Sakyo Ku, Kyoto, Japan
关键词
Listeria monocytogenes; most probable number (MPN); polymerase chain reaction (PCR); beef offal; prevalence; OUTBREAK;
D O I
10.1590/S1517-83822014005000002
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A total of 63 beef offal samples (beef liver = 16; beef lung = 14; beef intestine = 9; beef tripe = 15; beef spleen = 9) from three wet markets (A, B, and C) in Selangor, Malaysia were examined for the prevalence and microbial load of Listeria monocytogenes. A combination of the most probable number and polymerase chain reaction (MPN-PCR) method was employed in this study. It was found that L. monocytogenes detected in 33.33% of the beef offal samples. The prevalence of L. monocytogenes in beef offal purchased from wet markets A, B, and C were 22.73%, 37.50% and 41.18% respectively. The density of L. monocytogenes in all the samples ranged from < 3 up to > 2,400 MPN/g. The findings in this study indicate that beef offal can be a potential vehicle of foodborne listeriosis.
引用
收藏
页码:1169 / 1172
页数:4
相关论文
共 17 条
  • [1] PREVALENCE OF LISTERIA-MONOCYTOGENES IN FOODS IN MALAYSIA
    ARUMUGASWAMY, RK
    ALI, GRR
    HAMID, SNBA
    [J]. INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 1994, 23 (01) : 117 - 121
  • [2] PCR detection of Listeria monocytogenes:: a study of multiple factors affecting sensitivity
    Aznar, R
    Alarcón, B
    [J]. JOURNAL OF APPLIED MICROBIOLOGY, 2003, 95 (05) : 958 - 966
  • [3] Pathophysiology and epidemiology of listeriosis
    Berche, P
    [J]. BULLETIN DE L ACADEMIE NATIONALE DE MEDECINE, 2005, 189 (03): : 507 - 521
  • [4] Listeriosis: a primer
    Bortolussi, Robert
    [J]. CANADIAN MEDICAL ASSOCIATION JOURNAL, 2008, 179 (08) : 795 - 797
  • [5] BROOME CV, 1990, T IND MICR, P61
  • [6] Thermophilic Campylobacter spp. in salad vegetables in Malaysia
    Chai, Lay Ching
    Robin, Tunung
    Ragavan, Usha Menon
    Gunsalam, Jurin Wolmon
    Abu Bakar, Fatimah
    Ghazali, Farinazleen Mohamad
    Radu, Son
    Kumar, Malakar Pradeep
    [J]. INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2007, 117 (01) : 106 - 111
  • [7] An outbreak of gastroenteritis and fever due to Listeria monocytogenes in milk
    Dalton, CB
    Austin, CC
    Sobel, J
    Hayes, PS
    Bibb, WF
    Graves, LM
    Swaminathan, B
    Proctor, ME
    Griffin, PM
    [J]. NEW ENGLAND JOURNAL OF MEDICINE, 1997, 336 (02) : 100 - 105
  • [8] Endang Purwati Endang Purwati, 2003, Journal of Animal and Veterinary Advances, V2, P237
  • [9] Jeyaletchumi P., 2010, International Food Research Journal, V17, P1
  • [10] Identification, subtyping and virulence determination of Listeria monocytogenes, an important foodborne pathogen
    Liu, Dongyou
    [J]. JOURNAL OF MEDICAL MICROBIOLOGY, 2006, 55 (06) : 645 - 659