Plasmodium chabaudi: Expression of active recombinant chabaupain-1 and localization studies in Anopheles sp.

被引：15
作者：
Caldeira, Rubina L.
Goncalves, Lidia M. D. [3 ,4 ]
Martins, Tiago M.
Silveira, Henrique [2 ]
Novo, Carlos [2 ]
do Rosario, Virgilio [2 ]
Domingos, Ana [1 ,2 ]
机构：
[1] INETI Natl Inst Engn Technol & Innovat, Dept Biotechnol, Unit Prot & Monoclonal Antidody Technol, UTPAM, P-1649038 Lisbon, Portugal
[2] IHMT CMDT Inst Hyg & Trop Med, Ctr Malaria & Trop Dis, P-1349008 Lisbon, Portugal
[3] IBET Inst Biol Expt & Tecnol, P-2780901 Oeiras, Portugal
[4] Univ Lisbon, Fac Farm, IMed UL Res Inst Med & Pharmaceut Sci, P-1649003 Lisbon, Portugal
来源：
EXPERIMENTAL PARASITOLOGY | 2009年 / 122卷 / 02期
关键词：
Malaria; Plasmodium chabaudi; Recombinant cysteine proteases; Chabaupain; FALCIPARUM CYSTEINE PROTEASE; HEMOGLOBIN DEGRADATION; MALARIA; PROTEINASE; INHIBITION; DISRUPTION; ANTIBODY; PEPTIDE; CLONING; MICE;
D O I：
10.1016/j.exppara.2009.03.003
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
Plasmodium cysteine proteases have been shown to be immunogenic and are being used as malaria potential serodiagnostic markers and vaccine targets. Genes encoding two Plasmodium chabaudi cysteine proteases chabaupain-1 (CP-1) and chabaupain-2 (CP-2) were identified and further expressed in Escherichia coli. Solubilisation of recombinant CP-1 and CP-2 was achieved by decreasing the temperature of induction. Anopheles gambiae tissues infected with Plasmodium were analyzed by Western blotting using the anti-CP-1 antibody showing that CP-1 is only present in the A. gambiae midguts being absent from other infected mosquito biological material. Anti-CP-1 anti-serum recognized a 30 kDa band in P. chabaudi, Plasmodium berghei and Plasmodium yoelii lysates but does not recognize the recombinant CP-2 extracts suggesting high antibody specificity. (c) 2009 Elsevier Inc. All rights reserved.
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收藏
页码：97 / 105
页数：9
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