Evidence of immune and inflammatory processes in the gills of AGD-affected Atlantic salmon, Salmo salar L.

被引：50

作者：

Pennacchi, Y. ^{[1
]}

Leef, M. J. ^{[1
]}

Crosbie, P. B. B. ^{[1
]}

Nowak, B. F. ^{[1
]}

Bridle, A. R. ^{[1
]}

机构：

[1] Univ Tasmania, Australian Maritime Coll, Natl Ctr Marine Conservat & Resource Sustainabil, Launceston, Tas 7250, Australia

来源：

FISH & SHELLFISH IMMUNOLOGY | 2014年 / 36卷 / 02期

关键词：

AGD; Atlantic salmon; mRNA gene expression; 2D quantitative RT-PCR; inflammatory response; TROUT ONCORHYNCHUS-MYKISS; SPARUS-AURATA L; RAINBOW-TROUT; DISEASE AGD; NEOPARAMOEBA-PERURANS; ACIDOPHILIC GRANULOCYTES; EXPRESSION; INFECTION; PATHOLOGY; GENES;

D O I：

10.1016/j.fsi.2013.12.013

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

Amoebic gill disease (AGD) is a disease caused by the ectoparasite Neoparamoeba perurans which affects several cultured marine fish worldwide. The characterisation of pro-inflammatory and immune related genes at the mRNA level in AGD-affected Atlantic salmon gills was performed at 10 days post-inoculation using 2D quantitative RT-PCR, a method of mapping transcriptional responses in tissues. The genes of interest were IL-1 beta, TNF-alpha, TCR-alpha chain, CD8, CD4, MHC-II alpha, MHC-I, IgM and IgT. A significant increase in expression of the mRNA of all the genes was observed in the gills of AGD-affected fish. Contrary to previous studies, our data suggest that the parasite, N. perurans, elicits a classical inflammatory response in the gills of AGD-affected fish and indicates that the mRNA expression of immune genes within gill lesions misrepresents the cellular immune response in the gills during AGD. (C) 2014 Elsevier Ltd. All rights reserved.

引用

页码：563 / 570

页数：8

共 48 条

[1] Gross pathology and its relationship with histopathology of amoebic gill disease (AGD) in farmed Atlantic salmon, Salmo salar L. [J].