Chimaeric Rift Valley Fever Virus-Like Particle Vaccine Candidate Production in Nicotiana benthamiana

被引：13
作者：
Mbewana, Sandiswa [1 ]
Meyers, Ann E. [1 ]
Rybicki, Edward P. [1 ,2 ]
机构：
[1] Univ Cape Town, Dept Mol & Cell Biol, Biopharming Res Unit, Private Bag X3,22 Univ Ave, ZA-7700 Cape Town, South Africa
[2] Univ Cape Town, Fac Hlth Sci, Inst Infect Dis & Mol Med, Anzio Rd, ZA-7925 Cape Town, South Africa
来源：
BIOTECHNOLOGY JOURNAL | 2019年 / 14卷 / 04期
基金：
新加坡国家研究基金会;
关键词：
agroinfiltration; chimaeric virus-like particles; Nicotiana benthamiana; Rift Valley fever; vaccine; ENVELOPE GLYCOPROTEINS; IMMUNE-RESPONSES; DISEASE VIRUS; EXPRESSION; PLANTS; PROTEINS; GENE; ORGANIZATION; REPLICATION; MATURATION;
D O I：
10.1002/biot.201800238
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Rift Valley fever virus (RVFV) is an emerging mosquito-borne virus and hemorrhagic fever agent, which causes abortion storms in farmed small ruminants and potentially causes miscarriages in humans. Although live-attenuated vaccines are available for animals, they can only be used in endemic areas and there are currently no commercially available vaccines for humans. Here the authors describe the production of chimaeric RVFV virus-like particles transiently expressed in Nicotiana benthamiana by Agrobacterium tumefaciens-mediated gene transfer. The glycoprotein (Gn) gene is modified by removing its ectodomain (Gne) and fusing it to the transmembrane domain and cytosolic tail-encoding region of avian influenza H5N1 hemagglutinin. This is expressed transiently in N. benthamiana with purified protein yields calculated to be approximate to 57 mg kg(-1) fresh weight. Transmission electron microscopy shows putative chimaeric RVFV Gne-HA particles of 49-60 nm which are immunogenic, eliciting Gn-specific antibody responses in vaccinated mice without the use of adjuvant. To our knowledge, this is the first demonstration of the synthesis of Gne-HA chimaeric RVFV VLPs and the first demonstration of a detectable yield of RVFV Gn in plants.
引用
收藏
页数：9
相关论文
共 54 条
[31]   A replication-incompetent Rift Valley fever vaccine: Chimeric virus-like particles protect mice and rats against lethal challenge [J].
Mandell, Robert B. ;
Koukuntla, Ramesh ;
Mogler, Laura J. K. ;
Carzoli, Andrea K. ;
Freiberg, Alexander N. ;
Holbrook, Michael R. ;
Martin, Brian K. ;
Staplin, William R. ;
Vahanian, Nicholas N. ;
Link, Charles J. ;
Flick, Ramon .
VIROLOGY, 2010, 397 (01) :187-198
[32]   Production of H5N1 Influenza Virus Matrix Protein 2 Ectodomain Protein Bodies in Tobacco Plants and in Insect Cells as a Candidate Universal Influenza Vaccine [J].
Mbewana, Sandiswa ;
Mortimer, Elizabeth ;
Pera, Francisco F. P. G. ;
Hitzeroth, Inga Isabel ;
Rybicki, Edward P. .
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY, 2015, 3
[33]   Newcastle disease virus (NDV) marker vaccine:: an immunodominant epitope on the nucleoprotein gene of NDV can be deleted or replaced by a foreign epitope [J].
Mebatsion, T ;
Koolen, MJM ;
de Vaan, LTC ;
de Haas, N ;
Braber, M ;
Römer-Oberdörfer, A ;
van den Elzen, P ;
van der Marel, P .
JOURNAL OF VIROLOGY, 2002, 76 (20) :10138-10146
[34]   Vaccination with virus-like particles protects mice from lethal infection of Rift Valley Fever Virus [J].
Naslund, Jonas ;
Lagerqvist, Nina ;
Habjan, Matthias ;
Lundkvist, Ake ;
Evander, Magnus ;
Ahlm, Clas ;
Weber, Friedemann ;
Bucht, Goran .
VIROLOGY, 2009, 385 (02) :409-415
[35]   A Single Vaccination with an Improved Nonspreading Rift Valley Fever Virus Vaccine Provides Sterile Immunity in Lambs [J].
Oreshkova, Nadia ;
van Keulen, Lucien ;
Kant, Jet ;
Moormann, Rob J. M. ;
Kortekaas, Jeroen .
PLOS ONE, 2013, 8 (10)
[36]   Priming of strong, broad, and long-lived HIV type 1 p55gag-specific CD8+ cytotoxic T cells after administration of a virus-like particle vaccine in rhesus macaques [J].
Paliard, X ;
Liu, Y ;
Wagner, R ;
Wolf, H ;
Baenziger, J ;
Walker, CM .
AIDS RESEARCH AND HUMAN RETROVIRUSES, 2000, 16 (03) :273-282
[37]   Virus-Like Particles Expressing the Nucleocapsid Gene as an Efficient Vaccine Against Rift Valley Fever Virus [J].
Pichlmair, Andreas ;
Habjan, Matthias ;
Unger, Hermann ;
Weber, Friedemann .
VECTOR-BORNE AND ZOONOTIC DISEASES, 2010, 10 (07) :701-703
[38]   Extremely High-Level and Rapid Transient Protein Production in Plants without the Use of Viral Replication [J].
Sainsbury, Frank ;
Lomonossoff, George P. .
PLANT PHYSIOLOGY, 2008, 148 (03) :1212-1218
[39]   pEAQ: versatile expression vectors for easy and quick transient expression of heterologous proteins in plants [J].
Sainsbury, Frank ;
Thuenemann, Eva C. ;
Lomonossoff, George P. .
PLANT BIOTECHNOLOGY JOURNAL, 2009, 7 (07) :682-693
[40]  
Sainsbury Frank, 2009, V483, P25, DOI 10.1007/978-1-59745-407-0_2
123456