Intestinal inflammation modulates expression of the synaptic vesicle protein neuronal calcium sensor-1

被引:12
作者
Lourenssen, S
Jeromin, A
Roder, J
Blennerhassett, MG
机构
[1] Queens Univ, Hotel Dieu Hosp, Gastrointestinal Dis Res Unit, Kingston, ON K7L 5G2, Canada
[2] Mt Sinai Hosp, Toronto, ON M5G 1X5, Canada
来源
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY | 2002年 / 282卷 / 06期
关键词
frequenin; enteric nervous system; dinitrobenzene sulfonic acid;
D O I
10.1152/ajpgi.00320.2001
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
The calcium-binding protein neuronal calcium sensor 1 (NCS-1) is involved in modulation of neurotransmitter release in the peripheral and central nervous systems. Since intestinal inflammation impairs neurotransmitter release, we evaluated the expression of NCS-1 in the normal rat colon and in dinitrobenzene sulfonic acid (DNBS)-induced colitis. Immunocytochemistry and Western blots showed high levels of NCS-1 in the myenteric plexus and in axons in the smooth muscle layers; 23 +/- 2% of myenteric neurons were NCS-1 positive, with staining restricted to the largest neurons. NCS-1-positive axons decreased to 13.3 +/- 0.4% of total axons by day 2 and dropped further to 7.0 +/- 0.1% by day 4, returning to control levels by day 16. Dual-label Western blot analysis showed that the expression of NCS-1 relative to PGP 9.5 decreased by 50% on day 4 but returned to control by day 16. The selective loss of NCS-1 during colitis may underlie the altered neural function seen in the inflamed intestine.
引用
收藏
页码:G1097 / G1104
页数:8
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