Characteristics of internalization of NMDA-type GluRs with antibodies to GluN1 and GluN2B

被引：2
|
作者：
Takahashi, Yukitoshi [1 ,2 ,3 ]
Nishimura, Shigeko [1 ]
Takao, Emiko [1 ]
Kasai, Risa [1 ]
Enokida, Kaoru [1 ]
Ida, Kuniko [1 ]
Fukuoka, Masataka [1 ]
Koike, Takayoshi [1 ]
Omatsu, Hiroo [1 ]
Yamaguchi, Tokito [1 ]
Takano, Shiho [4 ,5 ]
Yoshida, Tomoyuki [4 ,5 ]
Mori, Hisashi [4 ,5 ]
机构：
[1] Natl Epilepsy Ctr, Shizuoka Inst Epilepsy & Neurol Disorders, NHO, 886 Urushiyama,Aoi Ward, Shizuoka 4208688, Japan
[2] Gifu Univ, Dept Pediat, Sch Med, Gifu, Japan
[3] Univ Shizuoka, Sch Pharmaceut Sci, Shizuoka, Japan
[4] Toyama Univ, Dept Mol Neurosci, Grad Sch Innovat Life Sci, Toyama, Japan
[5] Toyama Univ, Dept Mol Neurosci, Grad Sch Med, Toyama, Japan
来源：
JOURNAL OF NEUROIMMUNOLOGY | 2020年 / 349卷
关键词：
NMDA-type GluR; GluN1; GluN2B; Antibodies; Internalization; D-ASPARTATE RECEPTOR;
D O I：
10.1016/j.jneuroim.2020.577427
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
To characterize internalization of NMDA-type glutamate receptors (GluRs) by antibodies to NMDA-type GluRs, we produced rabbit antibodies to N-terminals of human GluN1 and GluN2B, and examined internalization of NMDA-type GluRs in HEK293T cells using confocal microscopy. Internalization of NMDA-type GluRs occurred from at least 10 min after incubation with antibodies to GluN1 and or GluN2B and was temperature-dependent. These findings confirm that antibodies to N-terminals of GluN1 and GluN2B present in the cerebrospinal fluid of patients with NMDAR encephalitis can mediate prompt internalization of NMDA-type GluR complexes.
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页数：10
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