Characteristics of internalization of NMDA-type GluRs with antibodies to GluN1 and GluN2B

被引：2

作者：

Takahashi, Yukitoshi ^{[1
,2
,3
]}

Nishimura, Shigeko ^{[1
]}

Takao, Emiko ^{[1
]}

Kasai, Risa ^{[1
]}

Enokida, Kaoru ^{[1
]}

Ida, Kuniko ^{[1
]}

Fukuoka, Masataka ^{[1
]}

Koike, Takayoshi ^{[1
]}

Omatsu, Hiroo ^{[1
]}

Yamaguchi, Tokito ^{[1
]}

Takano, Shiho ^{[4
,5
]}

Yoshida, Tomoyuki ^{[4
,5
]}

Mori, Hisashi ^{[4
,5
]}

机构：

[1] Natl Epilepsy Ctr, Shizuoka Inst Epilepsy & Neurol Disorders, NHO, 886 Urushiyama,Aoi Ward, Shizuoka 4208688, Japan

[2] Gifu Univ, Dept Pediat, Sch Med, Gifu, Japan

[3] Univ Shizuoka, Sch Pharmaceut Sci, Shizuoka, Japan

[4] Toyama Univ, Dept Mol Neurosci, Grad Sch Innovat Life Sci, Toyama, Japan

[5] Toyama Univ, Dept Mol Neurosci, Grad Sch Med, Toyama, Japan

来源：

JOURNAL OF NEUROIMMUNOLOGY | 2020年 / 349卷

关键词：

NMDA-type GluR; GluN1; GluN2B; Antibodies; Internalization; D-ASPARTATE RECEPTOR;

D O I：

10.1016/j.jneuroim.2020.577427

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

To characterize internalization of NMDA-type glutamate receptors (GluRs) by antibodies to NMDA-type GluRs, we produced rabbit antibodies to N-terminals of human GluN1 and GluN2B, and examined internalization of NMDA-type GluRs in HEK293T cells using confocal microscopy. Internalization of NMDA-type GluRs occurred from at least 10 min after incubation with antibodies to GluN1 and or GluN2B and was temperature-dependent. These findings confirm that antibodies to N-terminals of GluN1 and GluN2B present in the cerebrospinal fluid of patients with NMDAR encephalitis can mediate prompt internalization of NMDA-type GluR complexes.

引用

页数：10

共 50 条

[31] Age-Dependent, Subunit Specific Action of Hydrogen Sulfide on GluN1/2A and GluN1/2B NMDA Receptors
Yakovlev, Aleksey V.
Kurmasheva, Evgeniya D.
Ishchenko, Yevheniia
Giniatullin, Rashid
Sitdikova, Guzel F.
FRONTIERS IN CELLULAR NEUROSCIENCE, 2017, 11
[32] Different sites of alcohol action in the NMDA receptor GluN2A and GluN2B subunits
Zhao, Yulin
Ren, Hong
Dwyer, Donard S.
Peoples, Robert W.
NEUROPHARMACOLOGY, 2015, 97 : 240 - 250
[33] Negative allosteric modulation of GluN1/GluN3 NMDA receptors
Zhu, Zongjian
Yi, Feng
Epplin, Matthew P.
Liu, Ding
Summer, Samantha L.
Mizu, Ruth
Shaulsky, Gil
XiangWei, Wenshu
Tang, Weiting
Burger, Pieter B.
Menaldino, David S.
Myers, Scott J.
Liotta, Dennis C.
Hansen, Kasper B.
Yuan, Hongjie
Traynelis, Stephen F.
NEUROPHARMACOLOGY, 2020, 176
[34] Unmasking GluN1/GluN3A excitatory glycine NMDA receptors
Grand, Teddy
Gerges, Sarah Abi
David, Melissa
Diana, Marco A.
Paoletti, Pierre
NATURE COMMUNICATIONS, 2018, 9
[35] GluN2A and GluN2B subunit-containing NMDA receptors in hippocampal plasticity
Shipton, Olivia A.
Paulsen, Ole
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, 2014, 369 (1633)
[36] A single-channel mechanism for pharmacological potentiation of GluN1/GluN2A NMDA receptors
Chopra, Divyan A.
Sapkota, Kiran
Irvine, Mark W.
Fang, Guangyu
Jane, David E.
Monaghan, Daniel T.
Dravid, Shashank M.
SCIENTIFIC REPORTS, 2017, 7
[37] Pyrazole Inhibitors of GluN2B NMDA Receptor Subunit
Rosse, Gerard
ACS MEDICINAL CHEMISTRY LETTERS, 2016, 7 (12): : 1018 - 1019
[38] Protons Potentiate GluN1/GluN3A Glycinergic NMDA Receptor Currents
Cummings, Kirstie A.
Popescu, Gabriela K.
BIOPHYSICAL JOURNAL, 2015, 108 (02) : 285A - 285A
[39] Intersubunit interactions at putative sites of ethanol action in the M3 and M4 domains of the NMDA receptor GluN1 and GluN2B subunits
Zhao, Y.
Ren, H.
Peoples, R. W.
BRITISH JOURNAL OF PHARMACOLOGY, 2016, 173 (12) : 1950 - 1965
[40] Allosteric modulation of GluN1/GluN3 NMDA receptors by GluN1-selective competitive antagonists
Rouzbeh, Nirvan
Rau, Andrew R.
Benton, Avery J.
Yi, Feng
Anderson, Carly M.
Johns, Mia R.
Jensen, Loren
Lotti, James S.
Holley, David C.
Hansen, Kasper B.
JOURNAL OF GENERAL PHYSIOLOGY, 2023, 155 (06):

← 1 2 3 4 5 →