Characteristics of internalization of NMDA-type GluRs with antibodies to GluN1 and GluN2B

被引：2
|
作者：
Takahashi, Yukitoshi [1 ,2 ,3 ]
Nishimura, Shigeko [1 ]
Takao, Emiko [1 ]
Kasai, Risa [1 ]
Enokida, Kaoru [1 ]
Ida, Kuniko [1 ]
Fukuoka, Masataka [1 ]
Koike, Takayoshi [1 ]
Omatsu, Hiroo [1 ]
Yamaguchi, Tokito [1 ]
Takano, Shiho [4 ,5 ]
Yoshida, Tomoyuki [4 ,5 ]
Mori, Hisashi [4 ,5 ]
机构：
[1] Natl Epilepsy Ctr, Shizuoka Inst Epilepsy & Neurol Disorders, NHO, 886 Urushiyama,Aoi Ward, Shizuoka 4208688, Japan
[2] Gifu Univ, Dept Pediat, Sch Med, Gifu, Japan
[3] Univ Shizuoka, Sch Pharmaceut Sci, Shizuoka, Japan
[4] Toyama Univ, Dept Mol Neurosci, Grad Sch Innovat Life Sci, Toyama, Japan
[5] Toyama Univ, Dept Mol Neurosci, Grad Sch Med, Toyama, Japan
来源：
JOURNAL OF NEUROIMMUNOLOGY | 2020年 / 349卷
关键词：
NMDA-type GluR; GluN1; GluN2B; Antibodies; Internalization; D-ASPARTATE RECEPTOR;
D O I：
10.1016/j.jneuroim.2020.577427
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
To characterize internalization of NMDA-type glutamate receptors (GluRs) by antibodies to NMDA-type GluRs, we produced rabbit antibodies to N-terminals of human GluN1 and GluN2B, and examined internalization of NMDA-type GluRs in HEK293T cells using confocal microscopy. Internalization of NMDA-type GluRs occurred from at least 10 min after incubation with antibodies to GluN1 and or GluN2B and was temperature-dependent. These findings confirm that antibodies to N-terminals of GluN1 and GluN2B present in the cerebrospinal fluid of patients with NMDAR encephalitis can mediate prompt internalization of NMDA-type GluR complexes.
引用
收藏
页数：10
相关论文
共 50 条
  • [21] GluN2A and GluN2B NMDA receptors use distinct allosteric routes
    Meilin Tian
    David Stroebel
    Laura Piot
    Mélissa David
    Shixin Ye
    Pierre Paoletti
    Nature Communications, 12
  • [22] GluN2A and GluN2B NMDA receptors use distinct allosteric routes
    Tian, Meilin
    Stroebel, David
    Piot, Laura
    David, Melissa
    Ye, Shixin
    Paoletti, Pierre
    NATURE COMMUNICATIONS, 2021, 12 (01)
  • [23] Activation of Recombinant Rat GluN1/gluN2D NMDA Receptors
    Vance, Katie M.
    Hansen, Kasper B.
    Ogden, Kevin K.
    Traynelis, Stephen F.
    BIOPHYSICAL JOURNAL, 2010, 98 (03) : 525A - 525A
  • [24] Effects of Treadmill Exercise on Social Behavior in Rats Exposed to Thimerosal with Respect to the Hippocampal Level of GluN1, GluN2A, and GluN2B
    Alipour, Vahide
    Shabani, Ramin
    Rahmani-Nia, Farhad
    Vaseghi, Salar
    Nasehi, Mohammad
    Zarrindast, Mohammad-Reza
    JOURNAL OF MOLECULAR NEUROSCIENCE, 2022, 72 (06) : 1345 - 1357
  • [25] Effects of Treadmill Exercise on Social Behavior in Rats Exposed to Thimerosal with Respect to the Hippocampal Level of GluN1, GluN2A, and GluN2B
    Vahide Alipour
    Ramin Shabani
    Farhad Rahmani-Nia
    Salar Vaseghi
    Mohammad Nasehi
    Mohammad-Reza Zarrindast
    Journal of Molecular Neuroscience, 2022, 72 : 1345 - 1357
  • [26] MODIFYING ACUTE ETHANOL SENSITIVITY OF GLUN1/GLUN2B NMDA RECEPTORS VIA TRANSMEMBRANE DOMAIN MUTATION ALTERS SUSTAINED MECHANISMS OF ALCOHOL INHIBITION
    Hughes, B. A.
    Woodward, J. J.
    ALCOHOLISM-CLINICAL AND EXPERIMENTAL RESEARCH, 2015, 39 : 119A - 119A
  • [27] The differential contribution of GluN1 and GluN2 to the gating operation of the NMDA receptor channel
    Ya-Chi Tu
    Chung-Chin Kuo
    Pflügers Archiv - European Journal of Physiology, 2015, 467 : 1899 - 1917
  • [28] Unmasking GluN1/GluN3A excitatory glycine NMDA receptors
    Teddy Grand
    Sarah Abi Gerges
    Mélissa David
    Marco A. Diana
    Pierre Paoletti
    Nature Communications, 9
  • [29] The differential contribution of GluN1 and GluN2 to the gating operation of the NMDA receptor channel
    Tu, Ya-Chi
    Kuo, Chung-Chin
    PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 2015, 467 (09): : 1899 - 1917
  • [30] The GluN1/GluN2B NMDA receptor and metabotropic glutamate receptor 1 negative allosteric modulator has enhanced neuroprotection in a rat subarachnoid hemorrhage model
    Zhang, Zongyong
    Liu, Junke
    Fan, Cundong
    Mao, Leilei
    Xie, Rongxia
    Wang, Suyun
    Yang, Mingfeng
    Yuan, Hui
    Yang, Xiaoyi
    Sun, Jingyi
    Wang, Jian
    Kong, Jiming
    Huang, Siluo
    Sun, Baoliang
    EXPERIMENTAL NEUROLOGY, 2018, 301 : 13 - 25
12345