Characteristics of internalization of NMDA-type GluRs with antibodies to GluN1 and GluN2B

被引：2

作者：

Takahashi, Yukitoshi ^{[1
,2
,3
]}

Nishimura, Shigeko ^{[1
]}

Takao, Emiko ^{[1
]}

Kasai, Risa ^{[1
]}

Enokida, Kaoru ^{[1
]}

Ida, Kuniko ^{[1
]}

Fukuoka, Masataka ^{[1
]}

Koike, Takayoshi ^{[1
]}

Omatsu, Hiroo ^{[1
]}

Yamaguchi, Tokito ^{[1
]}

Takano, Shiho ^{[4
,5
]}

Yoshida, Tomoyuki ^{[4
,5
]}

Mori, Hisashi ^{[4
,5
]}

机构：

[1] Natl Epilepsy Ctr, Shizuoka Inst Epilepsy & Neurol Disorders, NHO, 886 Urushiyama,Aoi Ward, Shizuoka 4208688, Japan

[2] Gifu Univ, Dept Pediat, Sch Med, Gifu, Japan

[3] Univ Shizuoka, Sch Pharmaceut Sci, Shizuoka, Japan

[4] Toyama Univ, Dept Mol Neurosci, Grad Sch Innovat Life Sci, Toyama, Japan

[5] Toyama Univ, Dept Mol Neurosci, Grad Sch Med, Toyama, Japan

来源：

JOURNAL OF NEUROIMMUNOLOGY | 2020年 / 349卷

关键词：

NMDA-type GluR; GluN1; GluN2B; Antibodies; Internalization; D-ASPARTATE RECEPTOR;

D O I：

10.1016/j.jneuroim.2020.577427

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

To characterize internalization of NMDA-type glutamate receptors (GluRs) by antibodies to NMDA-type GluRs, we produced rabbit antibodies to N-terminals of human GluN1 and GluN2B, and examined internalization of NMDA-type GluRs in HEK293T cells using confocal microscopy. Internalization of NMDA-type GluRs occurred from at least 10 min after incubation with antibodies to GluN1 and or GluN2B and was temperature-dependent. These findings confirm that antibodies to N-terminals of GluN1 and GluN2B present in the cerebrospinal fluid of patients with NMDAR encephalitis can mediate prompt internalization of NMDA-type GluR complexes.

引用

页数：10

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