Characteristics of internalization of NMDA-type GluRs with antibodies to GluN1 and GluN2B

被引：2

作者：

Takahashi, Yukitoshi ^{[1
,2
,3
]}

Nishimura, Shigeko ^{[1
]}

Takao, Emiko ^{[1
]}

Kasai, Risa ^{[1
]}

Enokida, Kaoru ^{[1
]}

Ida, Kuniko ^{[1
]}

Fukuoka, Masataka ^{[1
]}

Koike, Takayoshi ^{[1
]}

Omatsu, Hiroo ^{[1
]}

Yamaguchi, Tokito ^{[1
]}

Takano, Shiho ^{[4
,5
]}

Yoshida, Tomoyuki ^{[4
,5
]}

Mori, Hisashi ^{[4
,5
]}

机构：

[1] Natl Epilepsy Ctr, Shizuoka Inst Epilepsy & Neurol Disorders, NHO, 886 Urushiyama,Aoi Ward, Shizuoka 4208688, Japan

[2] Gifu Univ, Dept Pediat, Sch Med, Gifu, Japan

[3] Univ Shizuoka, Sch Pharmaceut Sci, Shizuoka, Japan

[4] Toyama Univ, Dept Mol Neurosci, Grad Sch Innovat Life Sci, Toyama, Japan

[5] Toyama Univ, Dept Mol Neurosci, Grad Sch Med, Toyama, Japan

来源：

JOURNAL OF NEUROIMMUNOLOGY | 2020年 / 349卷

关键词：

NMDA-type GluR; GluN1; GluN2B; Antibodies; Internalization; D-ASPARTATE RECEPTOR;

D O I：

10.1016/j.jneuroim.2020.577427

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

To characterize internalization of NMDA-type glutamate receptors (GluRs) by antibodies to NMDA-type GluRs, we produced rabbit antibodies to N-terminals of human GluN1 and GluN2B, and examined internalization of NMDA-type GluRs in HEK293T cells using confocal microscopy. Internalization of NMDA-type GluRs occurred from at least 10 min after incubation with antibodies to GluN1 and or GluN2B and was temperature-dependent. These findings confirm that antibodies to N-terminals of GluN1 and GluN2B present in the cerebrospinal fluid of patients with NMDAR encephalitis can mediate prompt internalization of NMDA-type GluR complexes.

引用

页数：10

共 50 条

[11] Identification of a single amino acid in GluN1 that is critical for glycine-primed internalization of NMDA receptors
Han, Lu
Campanucci, Veronica A.
Cooke, James
Salter, Michael W.
MOLECULAR BRAIN, 2013, 6
[12] GluN2A versus GluN2B: twins, but quite different
Zhang, Xiao-Min
Luo, Jian-Hong
NEUROSCIENCE BULLETIN, 2013, 29 (06) : 761 - 772
[13] Mechanistic Insight into NMDA Receptor Dysregulation by Rare Variants in the GluN2A and GluN2B Agonist Binding Domains
Swanger, Sharon A.
Chen, Wenjuan
Wells, Gordon
Burger, Pieter B.
Tankovic, Anel
Bhattacharya, Subhrajit
Strong, Katie L.
Hu, Chun
Kusumoto, Hirofumi
Zhang, Jing
Adams, David R.
Millichap, John J.
Petrovski, Slave
Traynelis, Stephen F.
Yuan, Hongjie
AMERICAN JOURNAL OF HUMAN GENETICS, 2016, 99 (06) : 1261 - 1280
[14] Local constraints in either the GluN1 or GluN2 subunit equally impair NMDA receptor pore opening
Talukder, Iehab
Wollmuth, Lonnie P.
JOURNAL OF GENERAL PHYSIOLOGY, 2011, 138 (02) : 179 - 194
[15] Identification of a single amino acid in GluN1 that is critical for glycine-primed internalization of NMDA receptors
Lu Han
Verónica A Campanucci
James Cooke
Michael W Salter
Molecular Brain, 6
[16] Functional and pharmacological properties of triheteromeric GluN1/2B/2D NMDA receptors
Yi, Feng
Bhattacharya, Subhrajit
Thompson, Charles M.
Traynelis, Stephen F.
Hansen, Kasper B.
JOURNAL OF PHYSIOLOGY-LONDON, 2019, 597 (22): : 5495 - 5514
[17] Structural basis of subunit selectivity for competitive NMDA receptor antagonists with preference for GluN2A over GluN2B subunits
Lind, Genevieve E.
Mou, Tung-Chung
Tamborini, Lucia
Pomper, Martin G.
De Micheli, Carlo
Conti, Paola
Pinto, Andrea
Hansen, Kasper B.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2017, 114 (33) : E6942 - E6951
[18] Molecular basis of positive allosteric modulation of GluN2B NMDA receptors by polyamines
Mony, Laetitia
Zhu, Shujia
Carvalho, Stephanie
Paoletti, Pierre
EMBO JOURNAL, 2011, 30 (15) : 3134 - 3146
[19] Memory in aged mice is rescued by enhanced expression of the GluN2B subunit of the NMDA receptor
Brim, B. L.
Haskell, R.
Awedikian, R.
Ellinwood, N. M.
Jin, L.
Kumar, A.
Foster, T. C.
Magnusson, K. R.
BEHAVIOURAL BRAIN RESEARCH, 2013, 238 : 211 - 226
[20] Synaptic GluN2A and GluN2B Containing NMDA Receptors within the Superficial Dorsal Horn Activated following Primary Afferent Stimulation
Tong, Chi-Kun
MacDermott, Amy B.
JOURNAL OF NEUROSCIENCE, 2014, 34 (33) : 10808 - 10820

← 1 2 3 4 5 →