Single-cell RNA-seq reveals new types of human blood dendritic cells, monocytes, and progenitors

被引：1619

作者：

Villani, Alexandra-Chloe ^{[1
,2
]}

Satija, Rahul ^{[1
,3
,4
]}

Reynolds, Gary ^{[5
]}

Sarkizova, Siranush ^{[1
]}

Shekhar, Karthik ^{[1
]}

Fletcher, James ^{[5
]}

Griesbeck, Morgane ^{[6
]}

Butler, Andrew ^{[3
,4
]}

Zheng, Shiwei ^{[3
,4
]}

Lazo, Suzan ^{[7
]}

Jardine, Laura ^{[5
]}

Dixon, David ^{[5
]}

Stephenson, Emily ^{[5
]}

Nilsson, Emil ^{[8
]}

Grundberg, Ida ^{[8
]}

McDonald, David ^{[5
]}

Filby, Andrew ^{[5
]}

Li, Weibo ^{[1
,2
]}

De Jager, Philip L. ^{[9
,10
]}

Rozenblatt-Rosen, Orit ^{[1
]}

Lane, Andrew A. ^{[1
,7
]}

Haniffa, Muzlifah ^{[5
,11
]}

Regev, Aviv ^{[1
,12
,13
,14
]}

Hacohen, Nir ^{[1
,2
]}

机构：

[1] Broad Inst MIT & Harvard, Cambridge, MA USA

[2] Massachusetts Gen Hosp, Dept Med, Ctr Canc Res, Boston, MA 02114 USA

[3] NYU, Ctr Genom & Syst Biol, New York Genome Ctr, New York, NY USA

[4] NYU, Ctr Genom & Syst Biol, New York, NY USA

[5] Newcastle Univ, Inst Cellular Med, Newcastle Upon Tyne, Tyne & Wear, England

[6] Ragon Inst Massachusetts Gen Hosp Massachusetts I, Cambridge, MA USA

[7] Harvard Med Sch, Dana Farber Canc Inst, Dept Med Oncol, Boston, MA USA

[8] Olink Prote, Watertown, MA USA

[9] Brigham & Womens Hosp, Dept Neurol, Program Translat NeuroPsychiat Genom, Boston, MA 02115 USA

[10] Harvard Med Sch, Boston, MA USA

[11] Newcastle Hosp NHS Fdn Trust, Royal Victoria Infirm, Dept Dermatol, Newcastle Upon Tyne, Tyne & Wear, England

[12] MIT, Dept Biol, Cambridge, MA USA

[13] MIT, Koch Inst, 77 Massachusetts Ave, Cambridge, MA 02139 USA

[14] Howard Hughes Med Inst, Chevy Chase, MD USA

来源：

SCIENCE | 2017年 / 356卷 / 6335期

基金：

英国惠康基金;

关键词：

SUBSETS; DIFFERENTIATION; MACROPHAGES; REPRESENT; BIOLOGY; PATHWAY;

D O I：

10.1126/science.aah4573

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Dendritic cells (DCs) and monocytes play a central role in pathogen sensing, phagocytosis, and antigen presentation and consist of multiple specialized subtypes. However, their identities and interrelationships are not fully understood. Using unbiased single-cell RNA sequencing (RNA-seq) of ~2400 cells, we identified six human DCs and four monocyte subtypes in human blood. Our study reveals a new DC subset that shares properties with plasmacytoid DCs (pDCs) but potently activates T cells, thus redefining pDCs; a new subdivision within the CD1C+ subset of DCs; the relationship between blastic plasmacytoid DC neoplasia cells and healthy DCs; and circulating progenitor of conventional DCs (cDCs). Our revised taxonomy will enable more accurate functional and developmental analyses as well as immune monitoring in health and disease.

引用

页数：12

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